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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MA5-34637 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PRKD2 Recombinant Rabbit Monoclonal Antibody (JU08-33)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: A549, SH-SY-5Y, human tonsil tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JU08-33
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Protein Kinase D2 in A549 cell. Samples were incubated with Protein Kinase D2 monoclonal antibody (Product # MA5-34637), at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Protein Kinase D2 in SH-SY-5Y cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Protein Kinase D2 monoclonal antibody (Product # MA5-34637), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Protein Kinase D2 in A549 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Protein Kinase D2 monoclonal antibody (Product # MA5-34637), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Protein Kinase D2 in paraffin-embedded human tonsil tissue. Samples were incubated with Protein Kinase D2 monoclonal antibody (Product # MA5-34637), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Protein Kinase D2 in SH-SY-5Y cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with Protein Kinase D2 monoclonal antibody (Product # MA5-34637) at a dilution of 1:50, followed by Alexa Fluor 488-conjugated goat anti rabbit IgG.
