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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
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- Product number
- NB100-636 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-636, RRID:AB_2172684
- Product name
- Rabbit Polyclonal Protein Kinase D2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Tyrosine phosphorylation of protein kinase D2 mediates ligand-inducible elimination of the Type 1 interferon receptor.
Protein kinase D2 is a novel regulator of glioblastoma growth and tumor formation.
Ligand-stimulated downregulation of the alpha interferon receptor: role of protein kinase D2.
Role of the second cysteine-rich domain and Pro275 in protein kinase D2 interaction with ADP-ribosylation factor 1, trans-Golgi network recruitment, and protein transport.
Potent and selective disruption of protein kinase D functionality by a benzoxoloazepinolone.
The zinc-finger transcription factor, early growth response 3, mediates VEGF-induced angiogenesis.
Protein kinase D1 mediates NF-kappaB activation induced by cholecystokinin and cholinergic signaling in pancreatic acinar cells.
Dimeric PKD regulates membrane fission to form transport carriers at the TGN.
Zheng H, Qian J, Baker DP, Fuchs SY
The Journal of biological chemistry 2011 Oct 14;286(41):35733-41
The Journal of biological chemistry 2011 Oct 14;286(41):35733-41
Protein kinase D2 is a novel regulator of glioblastoma growth and tumor formation.
Azoitei N, Kleger A, Schoo N, Thal DR, Brunner C, Pusapati GV, Filatova A, Genze F, Möller P, Acker T, Kuefer R, Van Lint J, Baust H, Adler G, Seufferlein T
Neuro-oncology 2011 Jul;13(7):710-24
Neuro-oncology 2011 Jul;13(7):710-24
Ligand-stimulated downregulation of the alpha interferon receptor: role of protein kinase D2.
Zheng H, Qian J, Varghese B, Baker DP, Fuchs S
Molecular and cellular biology 2011 Feb;31(4):710-20
Molecular and cellular biology 2011 Feb;31(4):710-20
Role of the second cysteine-rich domain and Pro275 in protein kinase D2 interaction with ADP-ribosylation factor 1, trans-Golgi network recruitment, and protein transport.
Pusapati GV, Krndija D, Armacki M, von Wichert G, von Blume J, Malhotra V, Adler G, Seufferlein T
Molecular biology of the cell 2010 Mar 15;21(6):1011-22
Molecular biology of the cell 2010 Mar 15;21(6):1011-22
Potent and selective disruption of protein kinase D functionality by a benzoxoloazepinolone.
Sharlow ER, Giridhar KV, LaValle CR, Chen J, Leimgruber S, Barrett R, Bravo-Altamirano K, Wipf P, Lazo JS, Wang QJ
The Journal of biological chemistry 2008 Nov 28;283(48):33516-26
The Journal of biological chemistry 2008 Nov 28;283(48):33516-26
The zinc-finger transcription factor, early growth response 3, mediates VEGF-induced angiogenesis.
Liu D, Evans I, Britton G, Zachary I
Oncogene 2008 May 8;27(21):2989-98
Oncogene 2008 May 8;27(21):2989-98
Protein kinase D1 mediates NF-kappaB activation induced by cholecystokinin and cholinergic signaling in pancreatic acinar cells.
Yuan J, Lugea A, Zheng L, Gukovsky I, Edderkaoui M, Rozengurt E, Pandol SJ
American journal of physiology. Gastrointestinal and liver physiology 2008 Dec;295(6):G1190-201
American journal of physiology. Gastrointestinal and liver physiology 2008 Dec;295(6):G1190-201
Dimeric PKD regulates membrane fission to form transport carriers at the TGN.
Bossard C, Bresson D, Polishchuk RS, Malhotra V
The Journal of cell biology 2007 Dec 17;179(6):1123-31
The Journal of cell biology 2007 Dec 17;179(6):1123-31
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Protein Kinase D2 Antibody [NB100-636] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PKD2 antibody (lot For blotting immunoprecipitated PKD2, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Protein Kinase D2 Antibody [NB100-636] - Detection of Human PKD2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PKD2 antibody NB100-636 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Protein Kinase D2 Antibody [NB100-636] - Samples: Whole cell lysate (A. 30 ug/lane; B. 200 mcg/IP) from HEK293 cells that were untreated or transfected with expression constructs for PKD1 and PKD3 as controls for reactivity with these homologs. Antibodies: A. Affinity purified rabbit anti- NB100-636 used at the indicated oncentrations for WB. B. Affinity purified rabbit anti- NB100-636 and NB100-2299 used at 2 ug/200 mcg lysate for IP. Immunoprecipitated PKD2 was detected by WB using anti-PKD2 antibody NB100-2299 at 1 ug/ml. Detection: Chemiluminescence with a 10 second exposure.
