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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [2]
- Other assay [1]
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- Product number
- PA5-23653 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MRP3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.4 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references The Role of TRIP6, ABCC3 and CPS1 Expression in Resistance of Ovarian Cancer to Taxanes.
Overexpression of ABCC3 promotes cell proliferation, drug resistance, and aerobic glycolysis and is associated with poor prognosis in urinary bladder cancer patients.
Seborova K, Kloudova-Spalenkova A, Koucka K, Holy P, Ehrlichova M, Wang C, Ojima I, Voleska I, Daniel P, Balusikova K, Jelinek M, Kovar J, Rob L, Hruda M, Mrhalova M, Soucek P, Vaclavikova R
International journal of molecular sciences 2021 Dec 22;23(1)
International journal of molecular sciences 2021 Dec 22;23(1)
Overexpression of ABCC3 promotes cell proliferation, drug resistance, and aerobic glycolysis and is associated with poor prognosis in urinary bladder cancer patients.
Liu X, Yao D, Liu C, Cao Y, Yang Q, Sun Z, Liu D
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 Jun;37(6):8367-74
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 Jun;37(6):8367-74
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MRP3 in various lysates. Samples were incubated with MRP3 polyclonal antibody (Product # PA5-23653) using a dilution of 1:1,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Lane 1: Human brain lysate; Lane 2: Human testis lysate. Predicted band size: 169 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MRP3 in various lysates. Samples were incubated with MRP3 polyclonal antibody (Product # PA5-23653) using a dilution of 1:1,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Lane 1: HepG2 whole cell lysate; Lane 2: human liver lysate. Predicted band size: 169 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of MRP3 in formalin fixed and paraffin embedded human colon carcinoma. Samples were incubated with MRP3 polyclonal antibody (Product # PA5-23653) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of MRP3 in formalin fixed and paraffin embedded human colon carcinoma. Samples were incubated with MRP3 polyclonal antibody (Product # PA5-23653) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of MDA-MB435 cells using an ABCC3 polyclonal antibody (Product # PA5-23653) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of MRP3 in MDA-MB435 cells (right histogram). Samples were incubated with MRP3 polyclonal antibody (Product # PA5-23653) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (left histogram).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 ABCC3, CPS1, and TRIP6 expression in paclitaxel-resistant NCI/ADR-RES, SKOV-3/RES, and OVCAR-3/RES. ( A ) Bar graph showing relative expression of ABCC3, CPS1 and TRIP6 genes in paclitaxel-resistant ovarian cancer cell lines (technical triplicates). ( B ) Representative immunoblots of CPS1 in paclitaxel-resistant ovarian carcinoma cell lines. CPS1 silenced SKOV-3/RES cells or non-specific siRNA transfected SKOV-3/RES cells and human liver tissue were used as controls. ( C ) Representative immunoblot of ABCC3 in paclitaxel-resistant cell lines. ABCC3 silenced MCF-7/RES breast cancer cells or non-specific siRNA transfected MCF-7/PacR breast cancer cells and human liver tissue were used as controls. ( D ) Representative immunoblot of TRIP6 in paclitaxel-resistant ovarian carcinoma cell lines. beta-ACTIN served as a loading control. The size of TRIP6 band was confirmed previously by us [].
