Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-35941 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PBXIP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa.
- Concentration
- 1 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PBXIP1 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PBXIP1 Polyclonal Antibody (Product # PA5-35941) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PBXIP1 showing staining in the cytoplasm and nucleus of A431 cells. A431 cells were fixed in 4% paraformaldehyde/PBS for 15 min and stained using a PBXIP1 polyclonal antibody (Product # PA5-35941) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PBXIP1 Polyclonal Antibody detects PBXIP1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: PBXIP1 protein stained by PBXIP1 Polyclonal Antibody (Product # PA5-35941) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PBXIP1 Polyclonal Antibody detects PBXIP1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: PBXIP1 protein stained by PBXIP1 Polyclonal Antibody (Product # PA5-35941) diluted at 1:500. Blue: Hoechst 33342 staining.
