PA5-50527

antibody from Invitrogen Antibodies

Targeting: SLC1A5 AAAT, ASCT2, M7V1, RDRC

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-50527

Provider

Invitrogen Antibodies

Product name

ASCT2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

The antibody detects endogenous levels of total SLC1A5 / ASCT2 protein.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.2 mg/mL

Storage

-20°C

References

Submitted references

Integrated Metabolic and Epigenomic Reprograming by H3K27M Mutations in Diffuse Intrinsic Pontine Gliomas.

Chung C, Sweha SR, Pratt D, Tamrazi B, Panwalkar P, Banda A, Bayliss J, Hawes D, Yang F, Lee HJ, Shan M, Cieslik M, Qin T, Werner CK, Wahl DR, Lyssiotis CA, Bian Z, Shotwell JB, Yadav VN, Koschmann C, Chinnaiyan AM, Blüml S, Judkins AR, Venneti S
Cancer cell 2020 Sep 14;38(3):334-349.e9

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CD38-NAD(+)Axis Regulates Immunotherapeutic Anti-Tumor T Cell Response.

Chatterjee S, Daenthanasanmak A, Chakraborty P, Wyatt MW, Dhar P, Selvam SP, Fu J, Zhang J, Nguyen H, Kang I, Toth K, Al-Homrani M, Husain M, Beeson G, Ball L, Helke K, Husain S, Garrett-Mayer E, Hardiman G, Mehrotra M, Nishimura MI, Beeson CC, Bupp MG, Wu J, Ogretmen B, Paulos CM, Rathmell J, Yu XZ, Mehrotra S
Cell metabolism 2018 Jan 9;27(1):85-100.e8

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Slc1a5 was performed by loading (from left to right): Mouse lung tissue lysates (40µg) on to a 8% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a Slc1a5 antibody (Product # PA5-50527) at a 1/600 dilution for 10 minutes, followed by a secondary antibody at 1/8000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-ASCT2 Polyclonal Antibody (Product # PA5-50527) and a 57 kDa and 80 kDa band corresponding to Neutral amino acid transporter B(0) was observed across cell lines. Whole cell extracts (60 µg lysate) of MCF7 (Lane 1), HT-29 (Lane 2), A549 (Lane 3), T-47D (Lane 4), Hep G2 (Lane 5), BeWo (Lane 6), THP-1 (Lane 7) shown in figure a, and 3T3-L1 (Lane 8), 3T3-L1 (Lane 9) shown in fig b, were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # LC2002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Atto Ultimate Sensitivity Substrate (Product # A38556). 3T3L1 upon differentiation to adipocytes showed upregulation in both the canonical and glycosylated isoforms as shown in fig b. Fig c shows the densitometery for the upregulation of ASCT2 expression in differentiated adipocytes versus undifferentiated 3T3L1.