Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-78083 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NPAS1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: U87-MG, IMR32, SK-N-AS Predicted Reactivity: Bovine (94%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NPAS1 Polyclonal Antibody detects NPAS1 protein by Western blot analysis. A. 30 µg U87-MG whole cell lysate/extract. B. 30 µg IMR32 whole cell lysate/extract. C. 30 µg SK-N-AS whole cell lysate/extract.7.5 % SDS-PAGE. NPAS1 Polyclonal Antibody (Product # PA5-78083) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NPAS1 Polyclonal Antibody detects NPAS1 protein at nucleus by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: NPAS1 protein stained by NPAS1 Polyclonal Antibody (Product # PA5-78083) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NPAS1 Polyclonal Antibody detects NPAS1 protein at nucleus by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: NPAS1 protein stained by NPAS1 Polyclonal Antibody (Product # PA5-78083) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NPAS1 Polyclonal Antibody detects NPAS1 protein at nucleus on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. NPAS1 Polyclonal Antibody (Product # PA5-78083) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NPAS1 Polyclonal Antibody detects NPAS1 protein at nucleus on mouse middle brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse middle brain. NPAS1 Polyclonal Antibody (Product # PA5-78083) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NPAS1 Polyclonal Antibody detects NPAS1 protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. NPAS1 Polyclonal Antibody (Product # PA5-78083) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.