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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX113602 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX113602, RRID:AB_2037209
- Product name
- SAE1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Sumoylation is Required for the Cytoplasmic Accumulation of a Subset of mRNAs.
Zhang H, Mahadevan K, Palazzo AF
Genes 2014 Oct 20;5(4):982-1000
Genes 2014 Oct 20;5(4):982-1000
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE GTX113602 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SAE1 antibody detects SAE1 protein by Western blot analysis.A. 30 ?g Rat2 whole cell lysate/extract10 % SDS-PAGESAE1 antibody (GTX113602) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: A431 (GTX27909) B: H1299 10% SDS PAGE GTX113602 diluted at 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SAE1 antibody detects SAE1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SAE1 protein stained by SAE1 antibody (GTX113602) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of SAE1 protein from HepG2 whole cell extracts using 5 £gg of SAE1 antibody (GTX113602).Western blot analysis was performed using SAE1 antibody (GTX113602).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using SAE1(GTX113602) antibody at 1:100 dilution.
