AP09135PU-N

antibody from OriGene

Targeting: SAE1 AOS1, FLJ3091, Sua1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

AP09135PU-N

Provider

OriGene

Product name

SAE1 rabbit polyclonal antibody, Purified

Antibody type

Polyclonal

Description

SAE1 rabbit polyclonal antibody, Purified

Host

Rabbit

Conjugate

Unconjugated

Epitope

SAE1

Isotype

IgG

Antibody clone number

NULL

Vial size

500 µg

Concentration

5.0 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Coomassie-stained SDS-PAGE of GST-SAE1 recombinant protein (Panel A) and western blotting (Panel B) of HeLa WC lysate (lane 1) and purified recombinant GST-SAE1 (lane 2) are presented to show specificity of purified anti-SUMO Activating Enzyme (SAE1) antibody. The recombinant protein (with tag) ~60 kDa band present in 35 ug lysate (green, 800 nm channel) is indicated by the arrowhead. Lane 2 contains 50 ng of purified recombinant GST-SAE1 and lane 3 contains 300 ng of purified GST. Proteins were separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:2,000. Incubation was overnight at 4¡ã C followed by washes and reaction with a 1:10,000 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] MXHu for 45 min at room temperature. Molecular weight markers are shown for both the coomassie-stained gel and the western blot (lane M, red, 700 nm channel). IRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Validation comment

WB