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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB4352 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4352, RRID:AB_1580013
- Product name
- SAE1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of SAE1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2.
Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1.
Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex.
Okuma T, Honda R, Ichikawa G, Tsumagari N, Yasuda H
Biochemical and biophysical research communications 1999 Jan 27;254(3):693-8
Biochemical and biophysical research communications 1999 Jan 27;254(3):693-8
Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1.
Desterro JM, Rodriguez MS, Kemp GD, Hay RT
The Journal of biological chemistry 1999 Apr 9;274(15):10618-24
The Journal of biological chemistry 1999 Apr 9;274(15):10618-24
Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex.
Gong L, Li B, Millas S, Yeh ET
FEBS letters 1999 Apr 1;448(1):185-9
FEBS letters 1999 Apr 1;448(1):185-9
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The SAE1 polyclonal antibody (Cat # PAB4352) is used in Western blot to detect SAE1 in mouse heart tissue lysate .
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of SAE1 (arrow) using rabbit SAE1 polyclonal antibody (Cat # PAB4352). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SAE1 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with SAE1 polyclonal antibody (Cat # PAB4352) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
