AP09416PU-S

SAE1 antibody from Acris Antibodies GmbH

AOS1, FLJ3091, Sua1

Western blot

Supportive data in Antibodypedia

ELISA

Recommended by provider

Provider product page for AP09416PU-S

Antibody Data

Product number

AP09416PU-S

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP09416PU-S, RRID:AB_2035945

Product name

anti SAE1 / AOS1 pSer185

Provider product page

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to a region surrounding S185 of the human SUMO Activating Enzyme E1 protein

Reactivity

Human, Mouse, Rat, Bovine, Canine

Host

Rabbit

Isotype

IgG

Vial size

25 µl

Concentration

0.71 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using Rabbit-anti-SAE1 pS185 antibody shows detection of phosphorylated SAE1. Left lane (-) contains 20 µg human HeLa whole cell protein. Right lane (+) contains 20 µg human HeLa whole cell protein from cells pre-treated with phosphatase inhibitor cocktail to prevent dephosphorylation of the target. Proteins were separated on a 10% SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% milk-TBST 1 hr at room temperature, the membrane was probed with the primary antibody diluted to 1:1,000 at room temperature for 3 hr followed by washes and reaction with HRP-conjugated secondary and ECL imaging.