Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-29993 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Carboxypeptidase B1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: AsPC-1, mouse pancreas, rat pancreas, CPB1-transfected 293T.
- Concentration
- 2.74 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Carboxypeptidase B using 30 µg of HepG2 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Carboxypeptidase B polyclonal antibody (Product # PA5-29993) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Carboxypeptidase B1 was performed by separating 50 µg of mouse tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Carboxypeptidase B1 Polyclonal Antibody (Product # PA5-29993) at a dilution of 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Carboxypeptidase B1 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Carboxypeptidase B1 Polyclonal Antibody (Product # PA5-29993) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Carboxypeptidase B1 was performed by separating 50 µg of rat tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Carboxypeptidase B1 Polyclonal Antibody (Product # PA5-29993) at a dilution of 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Carboxypeptidase B1 was performed in paraffin-embedded mouse pancreas tissue using Carboxypeptidase B1 Polyclonal Antibody (Product # PA5-29993) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Carboxypeptidase B1 was performed in paraffin-embedded rat pancreas tissue using Carboxypeptidase B1 Polyclonal Antibody (Product # PA5-29993) at a dilution of 1:1000.
