PA5-29993

antibody from Invitrogen Antibodies

Targeting: CPB1

Antibody data

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Validation data

Product number: PA5-29993 - Provider product page
Provider: Invitrogen Antibodies
Product name: Carboxypeptidase B1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: AsPC-1, mouse pancreas, rat pancreas, CPB1-transfected 293T. Predicted reactivity: Rat (81%), Dog (86%), Pig (85%), Bovine (83%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 2.74 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

CPB1 protein structure - PA5-29993 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Carboxypeptidase B using 30 µg of HepG2 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Carboxypeptidase B polyclonal antibody (Product # PA5-29993) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Carboxypeptidase B1 was performed by separating 50 µg of mouse tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Carboxypeptidase B1 Polyclonal Antibody (Product # PA5-29993) at a dilution of 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of Carboxypeptidase B1 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Carboxypeptidase B1 Polyclonal Antibody (Product # PA5-29993) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Carboxypeptidase B1 was performed by separating 50 µg of rat tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Carboxypeptidase B1 Polyclonal Antibody (Product # PA5-29993) at a dilution of 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.