Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- ABIN951034 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Carboxypeptidase B1 (Tissue) (CPB1) (N-Term) antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 mg
Submitted references TAFI and pancreatic carboxypeptidase B modulate in vitro capillary tube formation by human microvascular endothelial cells.
Human procarboxypeptidase B: three-dimensional structure and implications for thrombin-activatable fibrinolysis inhibitor (TAFI).
Comparative analysis of the sequences and three-dimensional models of human procarboxypeptidases A1, A2 and B.
Guimarães AH, Laurens N, Weijers EM, Koolwijk P, van Hinsbergh VW, Rijken DC
Arteriosclerosis, thrombosis, and vascular biology 2007 Oct;27(10):2157-62
Arteriosclerosis, thrombosis, and vascular biology 2007 Oct;27(10):2157-62
Human procarboxypeptidase B: three-dimensional structure and implications for thrombin-activatable fibrinolysis inhibitor (TAFI).
Barbosa Pereira PJ, Segura-Martín S, Oliva B, Ferrer-Orta C, Avilés FX, Coll M, Gomis-Rüth FX, Vendrell J
Journal of molecular biology 2002 Aug 16;321(3):537-47
Journal of molecular biology 2002 Aug 16;321(3):537-47
Comparative analysis of the sequences and three-dimensional models of human procarboxypeptidases A1, A2 and B.
Aloy P, Catasús L, Villegas V, Reverter D, Vendrell J, Avilés FX
Biological chemistry 1998 Feb;379(2):149-55
Biological chemistry 1998 Feb;379(2):149-55
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of Carboxypeptidase B Antibody (N-term) Cat.-No AP51047PU-N in K562,Hela cell line lysates (35ug/lane).This demonstrates the CPB1 antibody detected the CPB1 protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of Carboxypeptidase B Antibody (N-term) Cat.-No AP51047PU-N with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Formalin fixed and paraffin embedded human pancreas tissue stained with Carboxypeptidase B Antibody (N-term) Cat.-No AP51047PU-Nfollowed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of CPB1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Hela cells using Carboxypeptidase B Antibody (N-term) Cat.-No AP51047PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.