Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-97189 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EBNA1BP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MDTPPLSDSE SESDESLVTD RELQDAFSRG LLKPGLNVVL EGPKKAVNDV NGLKQCLAEF KRDLEWVERL DVTLGPVPEI GGSEAPAPQN KDQKAVDPED DFQREMSFYR QAQAAVLAVL PRLHQLKVPT KRPTDYFAEM AKSDLQMQKI RQKLQTKQAA MERSEKAKQL RALRKYGKKV QTEVLQKRQQ EKAHMMNAIK KYQKGFSDKL DFLEGDQKPL AQRKKAGAKG QQMRKGPSAK RRYKNQKFGF GGKKKGSKWN TRESYDDVSS FRAKTAHGRG LKRPGKKGSN KRPGKRTREK MKNRTH; Positive Samples: A-431, LO2, HeLa, HT-1080, K-562, MCF7, Mouse pancreas; Cellular Location: Nucleus, nucleolus
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.65 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of EBNA1BP2 in extracts of various cell lines using EBNA1BP2 Polyclonal Antibody (Product # PA5-97189) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of EBNA1BP2 was performed in U2OS cells using EBNA1BP2 Polyclonal Antibody (Product # PA5-97189) at a dilution of 1:200. Blue: DAPI for nuclear staining.
