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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX114776 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX114776, RRID:AB_10624461
- Product name
- HLTF antibody [N2C1], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE GTX114776 diluted at 1:5000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HLTF(GTX114776) antibody at 1:500 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HLTF?antibody immunoprecipitates HLTF?protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 £gg HeLa whole cell lysate/extract B. Control with 2 £gg of preimmune rabbit IgG C. Immunoprecipitation of HLTF?protein by 2 £gg of HLTF?antibody (GTX114776) 7.5% SDS-PAGE The immunoprecipitated HLTF?protein was detected by HLTF?antibody (GTX114776) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded MB157 xenograft, using HLTF(GTX114776) antibody at 1:500 dilution.
