R32538

antibody from NSJ Bioreagents

Targeting: HLTF HIP116A, HLTF1, RNF80, SMARCA3, SNF2L3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

R32538

Provider

NSJ Bioreagents

Product name

HLTF Antibody / SMARCA3

Antibody type

Polyclonal

Description

This highly specific HLTF antibody is suitable for use in Western blot/Immunofluorescence/Flow cytometry applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the HLTF antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat heart, 2) mouse spleen and 3) human placenta lysate with HLTF antibody at 0.5ug/ml. Predicted/observed molecular weight: ~114 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human HeLa, 2) human PANC1, 3) human A549, 4) rat heart, 5) rat kidney, 6) mouse heart and 7) mouse kidney tissue lysate with HLTF antibody at 0.5ug/ml. Predicted molecular weight: ~114 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human A549 cells with HLTF antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human Caco-2 cells with HLTF antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HLTF antibody.