LS-C673583

antibody from LSBio

Targeting: HLTF HIP116A, HLTF1, RNF80, SMARCA3, SNF2L3

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C673583

Provider

LSBio

Product name

SMARCA3 / HLTF Antibody LS-C673583

Antibody type

Polyclonal

Description

Protein G purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Positive Western Blot detected in HepG2 whole cell lysate, K562 whole cell lysate. All lanes: HLTF antibody at 3 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 114 KDa. Observed band size: 114 KDa

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating HLTF in Hela whole cell lysate Lane 1: Rabbit control IgG instead of HLTF Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000) Lane 2: HLTF Antibody (6µg) + Hela whole cell lysate (1mg) Lane 3: Hela whole cell lysate (20µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating HLTF in Hela whole cell lysate Lane 1: Rabbit control IgG instead of HLTF Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000) Lane 2: HLTF Antibody (6µg) + Hela whole cell lysate (1mg) Lane 3: Hela whole cell lysate (20µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating HLTF in Hela whole cell lysate Lane 1: Rabbit control IgG instead of HLTF Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000) Lane 2: HLTF Antibody (6µg) + Hela whole cell lysate (1mg) Lane 3: Hela whole cell lysate (20µg)