AM02250PU-N

antibody from OriGene

Targeting: COL17A1 BP180, BPAG2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM02250PU-N

Provider

OriGene

Product name

Mouse monoclonal anti-Collagen type XVII alpha 1 chain (1-452) antibody, cloneV58, Purified

Antibody type

Monoclonal

Description

Mouse monoclonal anti-Collagen type XVII alpha 1 chain (1-452) antibody, cloneV58, Purified

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

Clone V58

Vial size

0.1mg

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western Blot analysis of Human Type XVII Collagen (Cat.-No. AM02250PU-N) specificity. Keratinocytes extracts were separated by SDS-PAGE and immunoblotted with Preimmune (Lane 1) and Immune Mouse serum (Lane 2) as well as with AM02250PU-N (Lane 3). AM02250PU-N detects a specific 180 kDa band.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

V-5-8 Monoclonal Antibody specifically binds to a 180 kDa protein by<strong> Immunoblotting:</strong> Different extracts of Human epidermis (Lanes 1-3) and primary Human keratinocytes (Lanes 4-11) were fractionated by 7.5% SDS-PAGE and immunoblotted with an 100-fold dilution of the Monoclonal antibody V-5-8. <br />Molecular weight markers are depicted to the right.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

V-5-8 Monoclonal Antibody labels the dermal-epidermal junction by Immunofluorescence Microscopy:?A 1/100 dilution of purified V-5-8 IgG was incubated 30 min at room temprature with Frozen Sections of human skin. After washing, bound IgG was visualized using FITC-conjugated antibodies specific for Mouse (B) IgG and (C) IgG2a (all magnification, x400).

Validation comment

IF