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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- RQ4497 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Collagen XVII Antibody / COL17A1
- Antibody type
- Monoclonal
- Description
- This highly specific Collagen XVII antibody is suitable for use in Immunohistochemistry/Western blot/Immunofluorescence applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- AA-3
- Vial size
- 100 ul
- Concentration
- Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA
- Storage
- Store the Collagen XVII antibody at -20oC.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human skin lysate with Collagen XVII antibody at 0.5ug/ml. Expected molecular weight ~120 kDa and ~180 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human skin lysate with Collagen XVII antibody at 0.5ug/ml. Expected molecular weight ~120 kDa and ~180 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) A431 and 2) HT-29 cell lysate with Collagen XVII antibody. Expected molecular weight ~120 kDa and ~180 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human PC-3 cell lysate with Collagen XVII antibody. Expected molecular weight ~120 kDa and ~180 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human A431 cells with Collagen XVII antibody (green) and DAPI nuclear stain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE mouse skin with Collagen XVII antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
