PA5-34548

antibody from Invitrogen Antibodies

Targeting: KAT5 cPLA2, ESA1, HTATIP, HTATIP1, PLIP, TIP60, ZC2HC5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

PA5-34548

Provider

Invitrogen Antibodies

Product name

TIP60 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

A suggested positive control is human brain tissue lysate. PA5-34548 can be used with blocking peptide PEP-1591.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

References

Submitted references

The Molecular Effects of Sulforaphane and Capsaicin on Metabolism upon Androgen and Tip60 Activation of Androgen Receptor.

Carrasco-Pozo C, Tan KN, Rodriguez T, Avery VM
International journal of molecular sciences 2019 Oct 29;20(21)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human brain tissue lysate using a KAT5 polyclonal antibody (Product # PA5-34548) at 1 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of KAT5 in human brain tissue lysate with TIP60 Polyclonal Antibody (Product # PA5-34548) at 1 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), FaDu (Lane 2), HCT 116 (Lane 3) and MCF-7 (Lane 4). The blot was probed with Anti-TIP60 Polyclonal Antibody (Product # PA5-34548, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 58.6 kDa band corresponding to TIP60 was observed across the cell lines tested (Lanes 1-4).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of TIP60 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with anti-TIP60 Rabbit Polyclonal Antibody (Product # PA5-34548) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing predominantly Nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.