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LearnPA5-29457
antibody from Invitrogen Antibodies
Targeting: KAT5
cPLA2, ESA1, HTATIP, HTATIP1, PLIP, TIP60, ZC2HC5
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
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- Product number
- PA5-29457 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TIP60 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Jurkat, Jurkat nuclear extract, mouse brain, Tip60 transfected 293T lysate. Predicted reactivity: Mouse (100%), Rat (100%), Pig (99%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.81 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TIP60 was performed by separating 30 µg of Jurkat whole cell and nuclear extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a TIP60 Polyclonal Antibody (Product # PA5-29457) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TIP60 Polyclonal Antibody (Product # PA5-29457). Sample (30 µg of whole cell lysate). Lane A: Non-transfected 293T lysate. Lane B: Tip60 transfected 293T lysate. TIP60 Polyclonal Antibody (Product # PA5-29457) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TIP60 Polyclonal Antibody detects KAT5 protein by western blot analysis. A. 50 µg mouse brain lysate/extract.7.5% SDS-PAGE. TIP60 Polyclonal Antibody (Product # PA5-29457) dilution: 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TIP60 in paraformaldehyde-fixed HeLa cells using a TIP60 polyclonal antibody (Product # PA5-29457) at a 1:200 dilution.
