PA5-29457

antibody from Invitrogen Antibodies

Targeting: KAT5 cPLA2, ESA1, HTATIP, HTATIP1, PLIP, TIP60, ZC2HC5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29457

Provider

Invitrogen Antibodies

Product name

TIP60 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: Jurkat, Jurkat nuclear extract, mouse brain, Tip60 transfected 293T lysate.

Concentration

0.81 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of TIP60 was performed by separating 30 µg of Jurkat whole cell and nuclear extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a TIP60 Polyclonal Antibody (Product # PA5-29457) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using TIP60 Polyclonal Antibody (Product # PA5-29457). Sample (30 µg of whole cell lysate). Lane A: Non-transfected 293T lysate. Lane B: Tip60 transfected 293T lysate. TIP60 Polyclonal Antibody (Product # PA5-29457) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

TIP60 Polyclonal Antibody detects KAT5 protein by western blot analysis. A. 50 µg mouse brain lysate/extract.7.5% SDS-PAGE. TIP60 Polyclonal Antibody (Product # PA5-29457) dilution: 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of TIP60 in paraformaldehyde-fixed HeLa cells using a TIP60 polyclonal antibody (Product # PA5-29457) at a 1:200 dilution.