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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-30347 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TOM1L1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, HeLa, TOM1L1-transfected 293T.
- Concentration
- 0.54 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TOM1L1 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a TOM1L1 Polyclonal Antibody (Product # PA5-30347) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TOM1L1 Polyclonal Antibody (Product # PA5-30347). Sample (30 µg of whole cell lysate). Lane A: A431. Lane B: HeLa. 10% SDS PAGE. TOM1L1 Polyclonal Antibody (Product # PA5-30347) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human hepatoma, using TOM1L1 (Product # PA5-30347) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
