PA1-41208

antibody from Invitrogen Antibodies

Targeting: SIK2 DKFZp434K1115, KIAA0781, LOH11CR1I, QIK, SNF1LK2

Western blot (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-41208

Provider

Invitrogen Antibodies

Product name

SIK2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Suggested positive control: human brain tissue, human brain protein.

Reactivity

Human, Mouse, Rat, Bovine, Canine

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Role of salt‑inducible kinase 2 in the malignant behavior and glycolysis of colorectal cancer cells.

Ni X, Feng Y, Fu X
Molecular medicine reports 2021 Nov;24(5)

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1. Expression level of SIK2 in CRC lines. (A) mRNA expression levels of SIK2 were notably increased in all types of cancer in the Cancer Cell Line Encyclopedia. The expression levels of SIK2 in the normal colonic epithelial cell line, NCM460, and different CRC cell lines (LoVo, SW480, SW620, CW2 and HCT116) were determined via (B) western blotting and (C) reverse transcription-quantitative PCR. ***P

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2. SIK2 silencing attenuates the proliferation, migration and invasion of colorectal cancer cells. The transfection efficiency in HCT116 cells transfected with siRNA-SIK2 was evaluated via (A) western blot and (B) reverse transcription-quantitative PCR analyses. (C) A Cell Counting Kit-8 assay was used to determine the proliferative ability of HCT116 cells. Cell migration and invasion rates were measured using (D) wound healing and (E) Transwell assays, respectively. Scale bar, 100 um. (F) MMP12 and MMP9 protein expression levels were detected using western blot analysis. ***P