Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- MA5-21268 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PWP1 Monoclonal Antibody (3A11)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Peptide Sequence: MNRSRQVTCV AWVRCGVAKE TPDKVELSKE EVKRLIAEAK EKLQEEGGGS DEEETGSPSE DGMQSARTQA RPREPLEDGD PEDDRTLDDD ELAEYDLDKY DEEGDPDAET LGESLLGLTV YGSNDQDPYV TLKDTEQYER EDFLIKPSDN LIVCGRAEQD QCNLDVHVYN QEEDSFYVHH DILLSAYPLS VEWLNFDPSP DDSTGNYIAV GNMTPVIEVW DLDIVDSLEP VFTLGSKLSK KKKKKGKKSS SAEGHTDAVL DLSWNKLIRN VLASASADNT VILWDMSLGK PAASLAVHTD KVQTLQFHPF EAQTLISGSY DKSVALYDCR SPDESHRMWR FSGQIERVTW NHFSPCHFLA STDDGFVYNL DARSDKPIFT LNAHNDEISG LDLSSQIKGC LVTASADKYV KIWDILGDRP SLVHSRDMKM GVLFCSSCCP DLPFIYALRG QKEGLRVWDI STVSSVNEAF GRRERLVLGS ARNSSISGPF GSRSSDTPME S
- Antibody clone number
- 3A11
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PWP1 expressed in transfected 293T cell line by anti-PWP1 monoclonal antibody (Product # MA5-21268), clone 3A11. Lane 1: PWP1 transfected lysate (Predicted MW: 55.9 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PWP1 expressed in transfected 293T cell line by anti-PWP1 monoclonal antibody (Product # MA5-21268), clone 3A11. Lane 1: PWP1 transfected lysate (Predicted MW: 55.9 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using an anti-PWP1 monoclonal antibody (Product # MA5-21268). (antibody concentration 10 µg/mL).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PWP1 using anti-PWP1 monoclonal antibody (Product # MA5-21268) is approximately 0.03 ng/mL as a capture antibody.