H00011137-M03

antibody from Abnova Corporation

Targeting: PWP1 IEF-SSP-9502

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00011137-M03

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00011137-M03, RRID:AB_1112433

Product name

PWP1 monoclonal antibody (M03), clone 1A12

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant PWP1.

Antigen sequence

MNRSRQVTCVAWVRCGVAKETPDKVELSKEEVKRL
IAEAKEKLQEEGGGSDEEETGSPSEDGMQSARTQA
RPREPLEDGDPEDDRTLDDDELAEYDLDKYDEEGD
PDAETLGESLLGLTVYGSNDQDPYVTLKDTEQYER
EDFLIKPSDNLIVCGRAEQDQCNLDVHVYNQEEDS
FYVHHDILLSAYPLSVEWLNFDPSPDDSTGNYIAV
GNMTPVIEVWDLDIVDSLEPVFTLGSKLSKKKKKK
GKKSSSAEGHTDAVLDLSWNKLIRNVLASASADNT
VILWDMSLGKPAASLAVHTDKVQTLQFHPFEAQTL
ISGSYDKSVALYDCRSPDESHRMWRFSGQIERVTW
NHFSPCHFLASTDDGFVYNLDARSDKPIFTLNAHN
DEISGLDLSSQIKGCLVTASADKYVKIWDILGDRP
SLVHSRDMKMGVLFCSSCCPDLPFIYALRGQKEGL
RVWDISTVSSVNEAFGRRERLVLGSARNSSISGPF
GSRSSDTPMES


Isotype

IgG

Antibody clone number

1A12

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of PWP1 expression in transfected 293T cell line by PWP1 monoclonal antibody (M03), clone 1A12.Lane 1: PWP1 transfected lysate (Predicted MW: 55.9 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged PWP1 is 0.3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol