Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- H00006879-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00006879-M01, RRID:AB_548532
- Product name
- Mouse Monoclonal TAF7 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. TAF7 - TAF7 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 55kDa
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Dynamic and regulated TAF gene expression during mouse embryonic germ cell development.
Core promoter factor TAF9B regulates neuronal gene expression.
Taf7l cooperates with Trf2 to regulate spermiogenesis.
Dual functions of TAF7L in adipocyte differentiation.
Core promoter recognition complex changes accompany liver development.
Gura MA, Mikedis MM, Seymour KA, de Rooij DG, Page DC, Freiman RN
PLoS genetics 2020 Jan;16(1):e1008515
PLoS genetics 2020 Jan;16(1):e1008515
Core promoter factor TAF9B regulates neuronal gene expression.
Herrera FJ, Yamaguchi T, Roelink H, Tjian R
eLife 2014 Jul 8;3:e02559
eLife 2014 Jul 8;3:e02559
Taf7l cooperates with Trf2 to regulate spermiogenesis.
Zhou H, Grubisic I, Zheng K, He Y, Wang PJ, Kaplan T, Tjian R
Proceedings of the National Academy of Sciences of the United States of America 2013 Oct 15;110(42):16886-91
Proceedings of the National Academy of Sciences of the United States of America 2013 Oct 15;110(42):16886-91
Dual functions of TAF7L in adipocyte differentiation.
Zhou H, Kaplan T, Li Y, Grubisic I, Zhang Z, Wang PJ, Eisen MB, Tjian R
eLife 2013 Jan 8;2:e00170
eLife 2013 Jan 8;2:e00170
Core promoter recognition complex changes accompany liver development.
D'Alessio JA, Ng R, Willenbring H, Tjian R
Proceedings of the National Academy of Sciences of the United States of America 2011 Mar 8;108(10):3906-11
Proceedings of the National Academy of Sciences of the United States of America 2011 Mar 8;108(10):3906-11
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TAF7 Antibody (2C5) [H00006879-M01] - Analysis of TAF7 over-expressed 293 cell line, cotransfected with TAF7 Validated Chimera RNAi ( Cat # H00006879-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TAF7 monoclonal antibody (M01), clone 2C5 (Cat # H00006879-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TAF7 Antibody (2C5) [H00006879-M01] - Analysis of TAF7 expression in transfected 293T cell line by TAF7 monoclonal antibody (M01), clone 2C5.Lane 1: TAF7 transfected lysate(40.3 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TAF7 Antibody (2C5) [H00006879-M01] - TAF7 monoclonal antibody (M01), clone 2C5 Analysis of TAF7 expression in MCF-7.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: TAF7 Antibody (2C5) [H00006879-M01] - Detection limit for recombinant GST tagged TAF7 is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: TAF7 Antibody (2C5) [H00006879-M01] - Analysis of monoclonal antibody to TAF7 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TAF7 Antibody (2C5) [H00006879-M01] - Analysis of monoclonal antibody to TAF7 on formalin-fixed paraffin-embedded human lymph node. Antibody concentration 3 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TAF7 Antibody (2C5) [H00006879-M01] - Analysis of monoclonal antibody to TAF7 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 1 ug/ml.