H00006879-M01

antibody from Novus Biologicals

Targeting: TAF7 TAF2F, TAFII55

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

H00006879-M01

Provider

Novus Biologicals

Proper citation

Novus Cat#H00006879-M01, RRID:AB_548532

Product name

Mouse Monoclonal TAF7 Antibody

Antibody type

Monoclonal

Description

IgG purified. TAF7 - TAF7 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 55kDa

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

Dynamic and regulated TAF gene expression during mouse embryonic germ cell development.

Gura MA, Mikedis MM, Seymour KA, de Rooij DG, Page DC, Freiman RN
PLoS genetics 2020 Jan;16(1):e1008515

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Core promoter factor TAF9B regulates neuronal gene expression.

Herrera FJ, Yamaguchi T, Roelink H, Tjian R
eLife 2014 Jul 8;3:e02559

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Taf7l cooperates with Trf2 to regulate spermiogenesis.

Zhou H, Grubisic I, Zheng K, He Y, Wang PJ, Kaplan T, Tjian R
Proceedings of the National Academy of Sciences of the United States of America 2013 Oct 15;110(42):16886-91

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Dual functions of TAF7L in adipocyte differentiation.

Zhou H, Kaplan T, Li Y, Grubisic I, Zhang Z, Wang PJ, Eisen MB, Tjian R
eLife 2013 Jan 8;2:e00170

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Core promoter recognition complex changes accompany liver development.

D'Alessio JA, Ng R, Willenbring H, Tjian R
Proceedings of the National Academy of Sciences of the United States of America 2011 Mar 8;108(10):3906-11

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: TAF7 Antibody (2C5) [H00006879-M01] - Analysis of TAF7 over-expressed 293 cell line, cotransfected with TAF7 Validated Chimera RNAi ( Cat # H00006879-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TAF7 monoclonal antibody (M01), clone 2C5 (Cat # H00006879-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: TAF7 Antibody (2C5) [H00006879-M01] - Analysis of TAF7 expression in transfected 293T cell line by TAF7 monoclonal antibody (M01), clone 2C5.Lane 1: TAF7 transfected lysate(40.3 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: TAF7 Antibody (2C5) [H00006879-M01] - TAF7 monoclonal antibody (M01), clone 2C5 Analysis of TAF7 expression in MCF-7.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: TAF7 Antibody (2C5) [H00006879-M01] - Detection limit for recombinant GST tagged TAF7 is approximately 0.3ng/ml as a capture antibody.

Immunocytochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: TAF7 Antibody (2C5) [H00006879-M01] - Analysis of monoclonal antibody to TAF7 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: TAF7 Antibody (2C5) [H00006879-M01] - Analysis of monoclonal antibody to TAF7 on formalin-fixed paraffin-embedded human lymph node. Antibody concentration 3 ug/ml.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: TAF7 Antibody (2C5) [H00006879-M01] - Analysis of monoclonal antibody to TAF7 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 1 ug/ml.