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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- PA5-30430 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PRDM4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: PC-3, U87-MG.
- Concentration
- 0.79 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PRDM4 Polyclonal Antibody (Product # PA5-30430). Sample (30 µg of whole cell lysate). Lane A: PC-3. Lane B: U87-MG . 7.5% SDS PAGE. PRDM4 Polyclonal Antibody (Product # PA5-30430) diluted at 1:5,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of PRDM4 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: PRDM4 Polyclonal Antibody (Product # PA5-30430) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PRDM4 Polyclonal Antibody detects PRDM4 protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PRDM4 protein stained by PRDM4 Polyclonal Antibody (Product # PA5-30430) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded mouse muscle, using PRDM4 (Product # PA5-30430) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using PRDM4 (Product # PA5-30430) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
