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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-B1642 - Provider product page
- Provider
- LSBio
- Product name
- Anti-NPM1 / NPM / Nucleophosmin Antibody (phospho-Thr199) IHC-plus™ LS-B1642
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50µl
- Storage
- +4°C or -20°C, Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Hela cells were treated with 300 M mimosine for 16 h, then placed in complete media (lane 1) or media containing 200 ng/ml nocodazole (lane 2) for an additional 18 h. Nuclear extract was western blotted with Poly6192. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Anti-NPM1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1642 dilution 1:50.
