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- Validations
- Western blot [6]
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- Immunohistochemistry [1]
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- Product number
- MA5-17141 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NPM1 Monoclonal Antibody (7H10B9)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-17141 targets Nucleophosmin in indirect ELISA, ICC, IHC, IF and WB applications and shows reactivity with Human and Non-human primate samples. The MA5-17141 immunogen is purified recombinant fragment of human NPM (2-265) expressed in E. Coli. MA5-17141 detects Nucleophosmin which has a predicted molecular weight of approximately 33kDa.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 7H10B9
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Treacle controls the nucleolar response to rDNA breaks via TOPBP1 recruitment and ATR activation.
Mooser C, Symeonidou IE, Leimbacher PA, Ribeiro A, Shorrocks AK, Jungmichel S, Larsen SC, Knechtle K, Jasrotia A, Zurbriggen D, Jeanrenaud A, Leikauf C, Fink D, Nielsen ML, Blackford AN, Stucki M
Nature communications 2020 Jan 8;11(1):123
Nature communications 2020 Jan 8;11(1):123
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Nucleophosmin using Nucleophosmin monoclonal antibody (Product # MA5-17141) in SMMC-7721 (1), HepG2 (2), HeLa (3) and HEK293 (4) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Nucleophosmin using Nucleophosmin monoclonal antibody (Product # MA5-17141) in SMMC-7721 (1), HepG2 (2), HeLa (3) and HEK293 (4) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Nucleophosmin using Nucleophosmin monoclonal antibody (Product # MA5-17141) in SMMC-7721 (1), HepG2 (2), HeLa (3) and HEK293 (4) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of NPM1 was achieved by transfecting HeLa with NPM1 specific siRNAs (Silencer® select Product # s9677, s9676). Western blot analysis (Fig. a) was performed using modified whole cell extracts (1% SDS) from NPM1 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with NPM1 Monoclonal Antibody (7H10B9) (Product # MA5-17141, 1:1500 dilution) and Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram where Fig. b represents the 32 kDa band Fig. c represents the 230 kDa band. Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to NPM1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-NPM1 Monoclonal Antibody (7H10B9) (Product # MA5-17141) and 29 kDa, 32 kDa and 230 kDa bands corresponding to NPM1 was observed across all the cell lines and tissues tested. Modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), COS-7 (Lane 2), C2C12 (Lane 3), HEK-293 (Lane 4), tissue extracts (30 ug lysate) of Mouse Liver (Lane 5) and Rat Brain (Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1500 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Nucleophosmin using Nucleophosmin monoclonal antibody (Product # MA5-17141) in SMMC-7721 (1), HepG2 (2), HeLa (3) and HEK293 (4) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa (left) and NTERA-2 (right) cells using Nucleophosmin monoclonal antibody (Product # MA5-17141) (Green). Red: Actin filaments have been labeled with DY-554 phalloidin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissues using Nucleophosmin monoclonal antibody (Product # MA5-17141) followed with DAB staining.
