10306-1-AP

antibody from Proteintech Group

Targeting: NPM1 B23, NPM

Provider product page for 10306-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [33]
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Validations
Western blot [1]
Immunohistochemistry [2]

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Product number: 10306-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#10306-1-AP, RRID:AB_2155163
Product name: B23/NPM1 antibody
Antibody type: Polyclonal
Description: B23/NPM1 antibody (Cat. #10306-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

NPM1 protein structure - 10306-1-AP shown in red.

Submitted references MANF Promotes Unexplained Recurrent Miscarriages by Interacting with NPM1 and Downregulating Trophoblast Cell Migration and Invasion.

Fang Y, Zhang J, Zhu D, Mei Q, Liao T, Cheng H, He Y, Cao Y, Wei Z
International journal of biological sciences 2024;20(1):296-311

NRDE2 deficiency impairs homologous recombination repair and sensitizes hepatocellular carcinoma to PARP inhibitors.

Wang Y, Liu X, Zuo X, Wang C, Zhang Z, Zhang H, Zeng T, Chen S, Liu M, Chen H, Song Q, Li Q, Yang C, Le Y, Xing J, Zhang H, An J, Jia W, Kang L, Zhang H, Xie H, Ye J, Wu T, He F, Zhang X, Li Y, Zhou G
Cell genomics 2024 May 8;4(5):100550

Rack1-mediated ferroptosis affects hindgut development in rats with anorectal malformations: Spatial transcriptome insights.

Wang CY, Li MY, Li SY, Wei XG, Dong NX, Liu ST, Yuan ZW, Li B, Pierro A, Tang XB, Bai YZ
Cell proliferation 2024 Jul;57(7):e13618

The characterization of protein lactylation in relation to cardiac metabolic reprogramming in neonatal mouse hearts.

Zhang T, Zhu Y, Wang X, Chong D, Wang H, Bu D, Zhao M, Fang L, Li C
Journal of genetics and genomics = Yi chuan xue bao 2024 Jul;51(7):735-748

AURKA inhibition induces Ewing's sarcoma apoptosis and ferroptosis through NPM1/YAP1 axis.

Chen H, Hu J, Xiong X, Chen H, Lin B, Chen Y, Li Y, Cheng D, Li Z
Cell death & disease 2024 Jan 29;15(1):99

Porcine circovirus type 2 infection promotes the SUMOylation of nucleophosmin-1 to facilitate the viral circular single-stranded DNA replication.

Du Q, Zhu L, Zhong J, Wei X, Zhang Q, Shi T, Han C, Yin X, Chen X, Tong D, Huang Y
PLoS pathogens 2024 Feb;20(2):e1012014

Cyclophilin A supports translation of intrinsically disordered proteins and affects haematopoietic stem cell ageing.

Maneix L, Iakova P, Lee CG, Moree SE, Lu X, Datar GK, Hill CT, Spooner E, King JCK, Sykes DB, Saez B, Di Stefano B, Chen X, Krause DS, Sahin E, Tsai FTF, Goodell MA, Berk BC, Scadden DT, Catic A
Nature cell biology 2024 Apr;26(4):593-603

NPM1 promotes nasopharyngeal carcinoma metastasis and stemness by recruiting Mdm2 to induce the ubiquitination-mediated degradation of p53.

Hu L, Gong P, Zhou N, Wang S, He C, Zhou Y, Li Q, Zhao X, Peng H
American journal of cancer research 2023;13(5):1766-1785

Immortalization-upregulated protein promotes pancreatic cancer progression by regulating NPM1/FHL1-mediated cell-cycle-checkpoint protein activity.

Luo Q, Pan Y, Fu Q, Zhang X, Zhou S, Yu P, Tian H, Liu P, Chen S, Zhang H, Qin T
Cell biology and toxicology 2023 Oct;39(5):2069-2087

Elevated high-mannose N-glycans hamper endometrial decidualization.

Chen S, Zhang A, Li N, Wu H, Li Y, Liu S, Yan Q
iScience 2023 Nov 17;26(11):108170

Nuclear PLD1 combined with NPM1 induces gemcitabine resistance through tumorigenic IL7R in pancreatic adenocarcinoma.

Fu D, Yan J, Zhang Z, Liu Y, Ma X, Ding J, Yang S, Zhao R, Chang A, Gao C, Liu J, Zhao T, Wang X, Huang C, Gao S, Ma Y, Tang B, Feng Y, Wang H, Hao J
Cancer biology & medicine 2023 Jun 27;20(8):599-626

Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges.

Miyagi T, Ueda K, Sugimoto M, Yagi T, Ito D, Yamazaki R, Narumi S, Hayamizu Y, Uji-I H, Kuroda M, Kanekura K
iScience 2023 Jun 16;26(6):106957

Disrupted phase behavior of FUS underlies poly-PR-induced DNA damage in amyotrophic lateral sclerosis.

Wang Y, Liu L, Chen H, Yang Y, Mu C, Ren H, Liu Y, Yu L, Fang Q, Wang G, Hao Z
Human molecular genetics 2023 Dec 12;33(1):64-77

Quantitative proteomics of HFD-induced fatty liver uncovers novel transcription factors of lipid metabolism.

Zhi S, Congcong Z, Zhiling G, Yihan Q, Yijing X, Guanjie L, Fang W, Xuehua S, Hongjie L, Xiaoni K, Yueqiu G
International journal of biological sciences 2022;18(8):3298-3312

Characterization and subcellular localization of Alongshan virus proteins.

Zhao Y, Wu P, Liu L, Ma B, Pan M, Huang Y, Du N, Yu H, Sui L, Wang ZD, Hou Z, Liu Q
Frontiers in microbiology 2022;13:1000322

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Burda JE, O'Shea TM, Ao Y, Suresh KB, Wang S, Bernstein AM, Chandra A, Deverasetty S, Kawaguchi R, Kim JH, McCallum S, Rogers A, Wahane S, Sofroniew MV
Nature 2022 Jun;606(7914):557-564

NPM1 promotes cell proliferation by targeting PRDX6 in colorectal cancer.

Wang D, Li Y, Liu Y, Cheng S, Liu F, Zuo R, Ding C, Shi S, Liu G
The international journal of biochemistry & cell biology 2022 Jun;147:106233

Lipophagy-ICAM-1 pathway associated with fatty acid and oxygen deficiencies is involved in poor prognoses of ovarian clear cell carcinoma.

Koizume S, Takahashi T, Nakamura Y, Yoshihara M, Ota Y, Sato S, Tadokoro H, Yokose T, Kato H, Miyagi E, Miyagi Y
British journal of cancer 2022 Aug;127(3):462-473

Nucleolar Phosphoprotein NPM1 Interacts With Porcine Circovirus Type 3 Cap Protein and Facilitates Viral Replication.

Song J, Hou L, Wang D, Wei L, Zhu S, Wang J, Quan R, Jiang H, Shi R, Liu J
Frontiers in microbiology 2021;12:679341

TRPV1 Activation Promotes β-arrestin2 Interaction with the Ribosomal Biogenesis Machinery in the Nucleolus:Implications for p53 Regulation and Neurite Outgrowth.

Hassan A, Iftinca M, Young D, Flynn R, Agosti F, Abdullah N, Defaye M, Scott MGH, Dufour A, Altier C
International journal of molecular sciences 2021 Feb 25;22(5)

RPL32 Promotes Lung Cancer Progression by Facilitating p53 Degradation.

Xie J, Zhang W, Liang X, Shuai C, Zhou Y, Pan H, Yang Y, Han W
Molecular therapy. Nucleic acids 2020 Sep 4;21:75-85

Nucleolar stress regulates stromal-epithelial transition via NPM1 during decidualization.

Liang YX, Hu W, Jin ZY, Diao HL, Liu L, Yang Y, Fu T, Yang ZM
Reproduction (Cambridge, England) 2020 Oct;160(4):491-500

Triptolide interrupts rRNA synthesis and induces the RPL23‑MDM2‑p53 pathway to repress lung cancer cells.

Wang J, Zhang ZQ, Li FQ, Chen JN, Gong X, Cao BB, Wang W
Oncology reports 2020 Jun;43(6):1863-1874

CircRNA WHSC1 targets the miR-646/NPM1 pathway to promote the development of endometrial cancer.

Liu Y, Chen S, Zong ZH, Guan X, Zhao Y
Journal of cellular and molecular medicine 2020 Jun;24(12):6898-6907

Subcellular localization of the porcine deltacoronavirus nucleocapsid protein.

Ding Z, Luo S, Gong W, Wang L, Ding N, Chen J, Chen J, Wang T, Ye Y, Song D, Kong L, Zhang J, Tang Y
Virus genes 2020 Dec;56(6):687-695

Global profiling of O-GlcNAcylated and/or phosphorylated proteins in hepatoblastoma.

Song H, Ma J, Bian Z, Chen S, Zhu J, Wang J, Huang N, Yin M, Sun F, Xu M, Pan Q
Signal transduction and targeted therapy 2019;4:40

Nucleolar stress regulation of endometrial receptivity in mouse models and human cell lines.

Hu W, Liang YX, Luo JM, Gu XW, Chen ZC, Fu T, Zhu YY, Lin S, Diao HL, Jia B, Yang ZM
Cell death & disease 2019 Nov 4;10(11):831

Excess hydrogen sulfide and polysulfides production underlies a schizophrenia pathophysiology.

Ide M, Ohnishi T, Toyoshima M, Balan S, Maekawa M, Shimamoto-Mitsuyama C, Iwayama Y, Ohba H, Watanabe A, Ishii T, Shibuya N, Kimura Y, Hisano Y, Murata Y, Hara T, Morikawa M, Hashimoto K, Nozaki Y, Toyota T, Wada Y, Tanaka Y, Kato T, Nishi A, Fujisawa S, Okano H, Itokawa M, Hirokawa N, Kunii Y, Kakita A, Yabe H, Iwamoto K, Meno K, Katagiri T, Dean B, Uchida K, Kimura H, Yoshikawa T
EMBO molecular medicine 2019 Dec;11(12):e10695

LNMICC Promotes Nodal Metastasis of Cervical Cancer by Reprogramming Fatty Acid Metabolism.

Shang C, Wang W, Liao Y, Chen Y, Liu T, Du Q, Huang J, Liang Y, Liu J, Zhao Y, Guo L, Hu Z, Yao S
Cancer research 2018 Feb 15;78(4):877-890

High expression of B23 is associated with tumorigenesis and poor prognosis in bladder urothelial carcinoma.

Wang H, Yuan G, Zhao B, Zhao Y, Qiu Y
Molecular medicine reports 2017 Feb;15(2):743-749

Protein expression of nucleophosmin, annexin A3 and nm23-H1 correlates with human nasopharyngeal carcinoma radioresistance in vivo.

Qu S, Li XY, Liang ZG, Li L, Huang ST, Li JQ, Li DR, Zhu XD
Oncology letters 2016 Jul;12(1):615-620

Proteomic characterization of the nucleolar linker histone H1 interaction network.

Szerlong HJ, Herman JA, Krause CM, DeLuca JG, Skoultchi A, Winger QA, Prenni JE, Hansen JC
Journal of molecular biology 2015 Jun 5;427(11):2056-71

Granulocyte colony-stimulating factor improves neuron survival in experimental spinal cord injury by regulating nucleophosmin-1 expression.

Guo Y, Liu S, Wang P, Zhang H, Wang F, Bing L, Gao J, Yang J, Hao A
Journal of neuroscience research 2014 Jun;92(6):751-60

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The B23 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human B23. This antibody recognizes human antigen. The B23 antibody has been validated for the following applications: ELISA, IHC, WB analysis.
Appendix: 10306-1-AP.pdf

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human colon cancer using 10306-1-AP(NPM1 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human colon cancer using 10306-1-AP(NPM1 antibody) at dilution of 1:100 (under 40x lens)
Sample type: tissue