60096-1-Ig

antibody from Proteintech Group

Targeting: NPM1 B23, NPM

Provider product page for 60096-1-Ig

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [58]
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Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

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Product number: 60096-1-Ig - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#60096-1-Ig, RRID:AB_2155162
Product name: B23/NPM1 antibody
Antibody type: Monoclonal
Description: KD/KO validated B23/NPM1 antibody (Cat. #60096-1-Ig) is a mouse monoclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Mouse
Conjugate: Unconjugated
Isotype: IgG
Antibody clone number: 4F12A3
Vial size: 20ul, 150ul

NPM1 protein structure - 60096-1-Ig shown in red.

Submitted references NPM1 phosphorylation-mediated telomere maintenance via stabilization of POLD3 in ALT-positive osteosarcoma: unraveling mechanisms and therapeutic opportunities.

Zhao R, Li T, Yang Q, Jiang D, Xue Y, Kou H, Wang Q, Wang Y, Han X, Ma W, Wang G, Feng J, Han X, Liu Y, Jing Y, Geng X, Wang F, Liu Y, Zhang Q, Wang F
Theranostics 2026;16(8):4224-4244

BARD1 recognizes pre-rRNA for DNA damage repair and rRNA biogenesis.

Huang H, Wu D, Yu X
The Journal of biological chemistry 2026 Mar 25;302(5):111406

Integrated multi-omics and functional analysis uncovers the structural and regulatory significance of Nucleophosmin 1 in the metabolic progression of esophageal carcinoma.

Liu XS, Chen X, Cheng ZM, Xie YC, Jiang T, Pei ZJ
International journal of biological macromolecules 2026 Jan;337(Pt 2):149610

DUX4-induced HSATII RNA accumulation drives protein aggregation, impacting RNA processing pathways.

Arends T, Bennett SR, Tapscott SJ
The Journal of cell biology 2026 Feb 2;225(2)

LIMK2 promotes centrosome clustering and cancer progression by activating MST4-mediated phosphorylation of NPM1.

Tian J, Liu S, Zhang Y, Jia H, Nie W, Yang R, Ge M, Liu K, Song M, Dong Z
Oncogene 2025 Oct;44(38):3625-3639

KIF18A promotes chromosome congression in cooperation with CENP-E downstream of CENP-C.

Miao J, Hara M, Su KC, Keys HR, Kong W, Takenoshita Y, Cheeseman IM, Fukagawa T
Cell reports 2025 Nov 25;44(11):116515

Mutant NPM1 modulates PDCD4 ubiquitination degradation and facilitates leukemogenesis.

Liang C, Ke J, Zhang Z, Guo H, Shang H, Liu D, Li S, Wu F
iScience 2025 Nov 21;28(11):113776

PKMYT1 kinase ameliorates cisplatin sensitivity in osteosarcoma.

Liu B, Li W, Zhang W, Feng C, Wan L, He S, Xu R, Fu Z, Liu Z, Xu H, Jin X, Tu C, Li Z
Signal transduction and targeted therapy 2025 May 21;10(1):165

Hypoxia upregulates the expression of PD-L1 via NPM1 in breast cancer.

Yu Y, Sun R, Hu F, Ding Z, Li X, Han J, Liang L, Wang T, Xi G, Dong X, Li Y, Zhao X, Zhang D
Journal for immunotherapy of cancer 2025 Jun 23;13(6)

Abnormal regulation of membrane-less organelles contributes to profilin1-associated ALS.

Ma G, Ruan X, Yang B, Li N, Su D, Sun S, Chen S, Xu K, Ying Z, Wang H
The Journal of biological chemistry 2025 Jul;301(7):110259

DUX4-induced HSATII RNA accumulation drives protein aggregation impacting RNA processing pathways.

Arends T, Bennett SR, Tapscott SJ
bioRxiv : the preprint server for biology 2025 Jan 22;

LINE1 and PRC2 control nucleolar organization and repression of the 8C state in human ESCs.

Zhang J, Ataei L, Mittal K, Wu L, Caldwell L, Huynh L, Sarajideen S, Tse K, Simon MM, Mazid MA, Cook DP, Trcka D, Kwan T, Hoffman MM, Wrana JL, Esteban MA, Ramalho-Santos M
Developmental cell 2025 Jan 20;60(2):186-203.e13

Generation of a Rat Monoclonal Antibody for Human Nucleophosmin.

Nishino Y, Ohshima N, Osaki T, Tachibana T, Yokoyama C
Monoclonal antibodies in immunodiagnosis and immunotherapy 2025 Feb;44(1):8-13

AURKB inhibition induces rhabdomyosarcoma apoptosis and ferroptosis through NPM1/SP1/ACSL5 axis.

Chen H, Li M, Zhang Y, Song M, Que Y, Wang J, Sun F, Zhu J, Huang J, Liu J, Xu J, Lu S, Zhang Y
JCI insight 2025 Feb 10;10(3)

SERPINB3 enhances NPM1 sumoylation via inhibiting SENP3's activity and promotes lung tumorigenesis.

Meng X, Wang W, Sheshadri N, Zhang T, Han X, Sun Y, Xiao M, Feng Y, Gao S, Chen J, Xie S, Wang Y, Yang Y, Zhao Y, Yin Y, Zong WX, Gao S
Cell death & disease 2025 Dec 24;17(1):133

SENP3 protects hepatocyte from pyroptosis during acute liver injury through deSUMOylation of HNRNPL.

Xiong X, Zhi Y, Yang N, Zhao W, Wang S, Zhu H, Tang J, Yi J, Sun X, Yang J
iScience 2025 Aug 15;28(8):113067

Targeting NPM1 inhibits proliferation and promotes apoptosis of hepatic progenitor cells via suppression of mTOR signalling pathway.

Wang P, Wang M, Liu L, Li H, Liu H, Ren J, Liu T, Cong M, Zhu Z, Zhao X, Sun L, Jia J
Stem cell research & therapy 2024 Sep 11;15(1):292

Nucleolin lactylation contributes to intrahepatic cholangiocarcinoma pathogenesis via RNA splicing regulation of MADD.

Yang L, Niu K, Wang J, Shen W, Jiang R, Liu L, Song W, Wang X, Zhang X, Zhang R, Wei D, Fan M, Jia L, Tao K
Journal of hepatology 2024 Oct;81(4):651-666

C9orf72 poly-PR forms anisotropic condensates causative of nuclear TDP-43 pathology.

Hodgson RE, Rayment JA, Huang WP, Sanchez Avila A, Ellis BCS, Lin YH, Soni N, Hautbergue GM, Shelkovnikova TA
iScience 2024 Oct 18;27(10):110937

Identification and functional analysis of a rare variant of gene DHX37 in a patient with 46,XY disorders of sex development.

Jiang W, Yu J, Mao Y, Tang Y, Cao L, Du Q, Li J, Yang J
Molecular genetics & genomic medicine 2024 May;12(5):e2453

A1CF Binding to the p65 Interaction Site on NKRF Decreased IFN-β Expression and p65 Phosphorylation (Ser536) in Renal Carcinoma Cells.

Liu Y, Yang J, Weng D, Xie Y
International journal of molecular sciences 2024 Mar 22;25(7)

Integrative single-cell and bulk transcriptomes analyses reveals heterogeneity of serine-glycine-one-carbon metabolism with distinct prognoses and therapeutic vulnerabilities in HNSCC.

Wang L, Yang R, Kong Y, Zhou J, Chen Y, Li R, Chen C, Tang X, Chen X, Xia J, Chen X, Cheng B, Ren X
International journal of oral science 2024 Jun 17;16(1):44

The characterization of protein lactylation in relation to cardiac metabolic reprogramming in neonatal mouse hearts.

Zhang T, Zhu Y, Wang X, Chong D, Wang H, Bu D, Zhao M, Fang L, Li C
Journal of genetics and genomics = Yi chuan xue bao 2024 Jul;51(7):735-748

Stress-induced TDP-43 nuclear condensation causes splicing loss of function and STMN2 depletion.

Huang WP, Ellis BCS, Hodgson RE, Sanchez Avila A, Kumar V, Rayment J, Moll T, Shelkovnikova TA
Cell reports 2024 Jul 23;43(7):114421

N6-methyladenosine-modified ALDH9A1 modulates lipid accumulation and tumor progression in clear cell renal cell carcinoma through the NPM1/IQGAP2/AKT signaling pathway.

Tan D, Miao D, Zhao C, Shi J, Lv Q, Lu F, Ruan H, Xiong Z, Zhang X
Cell death & disease 2024 Jul 22;15(7):520

Pumilio1 regulates NPM3/NPM1 axis to promote PD-L1-mediated immune escape in gastric cancer.

Wang H, Zhou Z, Zhang J, Hao T, Wang P, Wu P, Su R, Yang H, Deng G, Chen S, Gu L, He Y, Zeng L, Zhang C, Yin S
Cancer letters 2024 Jan 28;581:216498

Eukaryotic translation initiation factor 6-mediated ribosome biogenesis promotes synovial aggression and inflammation by increasing the translation of SP1 in rheumatoid arthritis.

Shen C, Peng C, Zhang S, Li R, Liu S, Kuang Y, Su F, Liu Y, Liang L, Xiao Y, Xu H
International immunopharmacology 2024 Dec 5;142(Pt B):113164

lncRNAs maintain the functional phase state of nucleolar prion-like protein to facilitate rRNA processing.

Sun YM, Zhu SX, Chen XT, Pan Q, An Y, Chen TQ, Huang HJ, Pu KJ, Lian JY, Zhao WL, Wang WT, Chen YQ
Molecular cell 2024 Dec 19;84(24):4878-4895.e10

Monitoring nucleolar-nucleoplasmic protein shuttling in living cells by high-content microscopy and automated image analysis.

Engbrecht M, Grundei D, Dilger AM, Wiedemann H, Aust AK, Baumgärtner S, Helfrich S, Kergl-Räpple F, Bürkle A, Mangerich A
Nucleic acids research 2024 Aug 27;52(15):e72

The effect of small molecule inhibitor NSC348884 on nucleophosmin 1-mutated acute myeloid leukemia cells.

Zhu JF, Han LL, Ma Y, Wang YX, Zhang F
European review for medical and pharmacological sciences 2023 Oct;27(19):9145-9151

Single-cell transcriptome analyses reveal critical roles of RNA splicing during leukemia progression.

Wu B, Chen X, Pan X, Deng X, Li S, Wang Z, Wang J, Liao D, Xu J, Chen M, Zhao C, Xue Z, Wang Y, Niu T, Lin J, Chen L, Liu Y, Chen C
PLoS biology 2023 May;21(5):e3002088

Alternative transcribed 3' isoform of long non-coding RNA Malat1 inhibits mouse retinal oxidative stress.

Ghanam AR, Ke S, Wang S, Elgendy R, Xie C, Wang S, Zhang R, Wei M, Liu W, Cao J, Zhang Y, Zhang Z, Xue T, Zheng Y, Song X
iScience 2023 Jan 20;26(1):105740

Heliangin acts as a covalent ligand of RPS2 that disrupts pre-rRNA metabolic processes in NPM1-mutated acute myeloid leukemia.

Feng Y, Han Y, Hu A, Qu Y, Hu Y, Wu H, Wang X, He L
Acta pharmaceutica Sinica. B 2023 Feb;13(2):598-617

Seven oxidative stress-related genes predict the prognosis of hepatocellular carcinoma.

Miao C, He X, Chen G, Kahlert UD, Yao C, Shi W, Su D, Hu L, Zhang Z
Aging 2023 Dec 14;15(24):15050-15063

GGC repeat expansion in NOTCH2NLC induces dysfunction in ribosome biogenesis and translation.

Fan Y, Li MJ, Yang J, Li SJ, Hao XY, Li JD, Wang YC, Tang MB, Zhang C, Shi JJ, Ma DR, Guo MN, Liu F, Shen S, Yao DB, Zuo CY, Mao CY, Hu ZW, Zhang S, Yang ZH, Guo GY, Yang JH, Xia ZP, Xu YM, Shi CH
Brain : a journal of neurology 2023 Aug 1;146(8):3373-3391

NOP53 undergoes liquid-liquid phase separation and promotes tumor radio-resistance.

Shi J, Chen SY, Shen XT, Yin XK, Zhao WW, Bai SM, Feng WX, Feng LL, Qin C, Zheng J, Wang YL, Fan XJ
Cell death discovery 2022 Oct 31;8(1):436

Downregulated RRS1 inhibits invasion and metastasis of BT549 through RPL11‑c‑Myc‑SNAIL axis.

Wang R, Peng C, Song J, Hua Y, Wu Q, Deng L, Cao Y, Zhang J, Zhang L, Wu L, Hou L
International journal of oncology 2022 Mar;60(3)

NPM1 promotes cell proliferation by targeting PRDX6 in colorectal cancer.

Wang D, Li Y, Liu Y, Cheng S, Liu F, Zuo R, Ding C, Shi S, Liu G
The international journal of biochemistry & cell biology 2022 Jun;147:106233

The binding between NPM and H2B proteins signals for the diabetes-associated centrosome amplification.

Xu SX, Han YW, Guo JL, Bian XK, Hu HM, Lee SC
Cell biochemistry and function 2022 Jul;40(5):516-525

Disruption of ER-mitochondria tethering and signalling in C9orf72-associated amyotrophic lateral sclerosis and frontotemporal dementia.

Gomez-Suaga P, Mórotz GM, Markovinovic A, Martín-Guerrero SM, Preza E, Arias N, Mayl K, Aabdien A, Gesheva V, Nishimura A, Annibali A, Lee Y, Mitchell JC, Wray S, Shaw C, Noble W, Miller CCJ
Aging cell 2022 Feb;21(2):e13549

Elevated FAM84B promotes cell proliferation via interacting with NPM1 in esophageal squamous cell carcinoma.

Wang F, Cheng C, Wang X, Chen F, Li H, Zhou Y, Wang Y, Hu X, Kong P, Zhang L, Cheng X, Cui Y
Cell death discovery 2022 Apr 8;8(1):182

Interactome screening of C9orf72 dipeptide repeats reveals VCP sequestration and functional impairment by polyGA.

Božič J, Motaln H, Janež AP, Markič L, Tripathi P, Yamoah A, Aronica E, Lee YB, Heilig R, Fischer R, Thompson AJ, Goswami A, Rogelj B
Brain : a journal of neurology 2022 Apr 18;145(2):684-699

Nuclear speckle specific hnRNP D-like prevents age- and AD-related cognitive decline by modulating RNA splicing.

Zhang Q, Zhang J, Ye J, Li X, Liu H, Ma X, Wang C, He K, Zhang W, Yuan J, Zhao Y, Xu H, Liu Q
Molecular neurodegeneration 2021 Sep 22;16(1):66

APOBEC1 complementation factor facilitates cell migration by promoting nucleus translocation of SMAD3 in renal cell carcinoma cells.

Xia H, Liu Y, Xu L, Mao H, Zhou Q, Xie Y
In vitro cellular & developmental biology. Animal 2021 May;57(5):501-509

HO-1 nuclear accumulation and interaction with NPM1 protect against stress-induced endothelial senescence independent of its enzymatic activity.

Luo W, Li J, Li Z, Lin T, Zhang L, Yang W, Mai Y, Liu R, Chen M, Dai C, Yang H, Lu J, Li H, Guan G, Huang M, Liu P, Li Z
Cell death & disease 2021 Jul 26;12(8):738

Genomic gain of RRS1 promotes hepatocellular carcinoma through reducing the RPL11-MDM2-p53 signaling.

Cao P, Yang A, Li P, Xia X, Han Y, Zhou G, Wang R, Yang F, Li Y, Zhang Y, Cui Y, Ji H, Lu L, He F, Zhou G
Science advances 2021 Aug;7(35)

Assessment of SENP3-interacting proteins in hepatocytes treated with diethylnitrosamine by BioID assay.

Chen F, Yan H, Guo C, Zhu H, Yi J, Sun X, Yang J
Acta biochimica et biophysica Sinica 2021 Aug 31;53(9):1237-1246

The protease activity of human ATG4B is regulated by reversible oxidative modification.

Zheng X, Yang Z, Gu Q, Xia F, Fu Y, Liu P, Yin XM, Li M
Autophagy 2020 Oct;16(10):1838-1850

Myc-mediated SDHA acetylation triggers epigenetic regulation of gene expression and tumorigenesis.

Li ST, Huang D, Shen S, Cai Y, Xing S, Wu G, Jiang Z, Hao Y, Yuan M, Wang N, Zhu L, Yan R, Yang D, Wang L, Liu Z, Hu X, Zhou R, Qu K, Li A, Duan X, Zhang H, Gao P
Nature metabolism 2020 Mar;2(3):256-269

Tight expression regulation of senataxin, linked to motor neuron disease and ataxia, is required to avert cell-cycle block and nucleolus disassembly.

Bennett CL, Sopher BL, La Spada AR
Heliyon 2020 Jun;6(6):e04165

MYCT1 represses apoptosis of laryngeal cancerous cells through the MAX/miR-181a/NPM1 pathway.

Wang HT, Tong X, Zhang ZX, Sun YY, Yan W, Xu ZM, Fu WN
The FEBS journal 2019 Oct;286(19):3892-3908

Identification of the key genes and pathways in prostate cancer.

Fan S, Liang Z, Gao Z, Pan Z, Han S, Liu X, Zhao C, Yang W, Pan Z, Feng W
Oncology letters 2018 Nov;16(5):6663-6669

Menin enhances c-Myc-mediated transcription to promote cancer progression.

Wu G, Yuan M, Shen S, Ma X, Fang J, Zhu L, Sun L, Liu Z, He X, Huang D, Li T, Li C, Wu J, Hu X, Li Z, Song L, Qu K, Zhang H, Gao P
Nature communications 2017 May 5;8:15278

Depletion of Dicer promotes epithelial ovarian cancer progression by elevating PDIA3 expression.

Zhu Y, Cai L, Guo J, Chen N, Yi X, Zhao Y, Cai J, Wang Z
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 Oct;37(10):14009-14023

A redox mechanism underlying nucleolar stress sensing by nucleophosmin.

Yang K, Wang M, Zhao Y, Sun X, Yang Y, Li X, Zhou A, Chu H, Zhou H, Xu J, Wu M, Yang J, Yi J
Nature communications 2016 Nov 25;7:13599

Nucleolar stress and impaired stress granule formation contribute to C9orf72 RAN translation-induced cytotoxicity.

Tao Z, Wang H, Xia Q, Li K, Li K, Jiang X, Xu G, Wang G, Ying Z
Human molecular genetics 2015 May 1;24(9):2426-41

Proteomic characterization of the nucleolar linker histone H1 interaction network.

Szerlong HJ, Herman JA, Krause CM, DeLuca JG, Skoultchi A, Winger QA, Prenni JE, Hansen JC
Journal of molecular biology 2015 Jun 5;427(11):2056-71

HP1-mediated formation of alternative lengthening of telomeres-associated PML bodies requires HIRA but not ASF1a.

Jiang WQ, Nguyen A, Cao Y, Chang AC, Reddel RR
PloS one 2011 Feb 15;6(2):e17036

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HepG2 cells were subjected to SDS PAGE followed by western blot with 60096-1-Ig(B23 antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of MCF-7 cells, using B23 antibody 60096-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human breast cancer using 60096-1-Ig(B23 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human breast cancer using 60096-1-Ig(B23 antibody) at dilution of 1:100 (under 40x lens)
Sample type: tissue