60096-1-Ig

antibody from Proteintech Group

Targeting: NPM1 B23, NPM

Provider product page for 60096-1-Ig

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [31]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

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Product number: 60096-1-Ig - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#60096-1-Ig, RRID:AB_2155162
Product name: B23/NPM1 antibody
Antibody type: Monoclonal
Description: KD/KO validated B23/NPM1 antibody (Cat. #60096-1-Ig) is a mouse monoclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Mouse
Conjugate: Unconjugated
Isotype: IgG
Antibody clone number: 4F12A3
Vial size: 20ul, 150ul

NPM1 protein structure - 60096-1-Ig shown in red.

Submitted references Nucleolin lactylation contributes to intrahepatic cholangiocarcinoma pathogenesis via RNA splicing regulation of MADD.

Yang L, Niu K, Wang J, Shen W, Jiang R, Liu L, Song W, Wang X, Zhang X, Zhang R, Wei D, Fan M, Jia L, Tao K
Journal of hepatology 2024 Oct;81(4):651-666

A1CF Binding to the p65 Interaction Site on NKRF Decreased IFN-β Expression and p65 Phosphorylation (Ser536) in Renal Carcinoma Cells.

Liu Y, Yang J, Weng D, Xie Y
International journal of molecular sciences 2024 Mar 22;25(7)

Pumilio1 regulates NPM3/NPM1 axis to promote PD-L1-mediated immune escape in gastric cancer.

Wang H, Zhou Z, Zhang J, Hao T, Wang P, Wu P, Su R, Yang H, Deng G, Chen S, Gu L, He Y, Zeng L, Zhang C, Yin S
Cancer letters 2024 Jan 28;581:216498

Monitoring nucleolar-nucleoplasmic protein shuttling in living cells by high-content microscopy and automated image analysis.

Engbrecht M, Grundei D, Dilger AM, Wiedemann H, Aust AK, Baumgärtner S, Helfrich S, Kergl-Räpple F, Bürkle A, Mangerich A
Nucleic acids research 2024 Aug 27;52(15):e72

Single-cell transcriptome analyses reveal critical roles of RNA splicing during leukemia progression.

Wu B, Chen X, Pan X, Deng X, Li S, Wang Z, Wang J, Liao D, Xu J, Chen M, Zhao C, Xue Z, Wang Y, Niu T, Lin J, Chen L, Liu Y, Chen C
PLoS biology 2023 May;21(5):e3002088

Alternative transcribed 3' isoform of long non-coding RNA Malat1 inhibits mouse retinal oxidative stress.

Ghanam AR, Ke S, Wang S, Elgendy R, Xie C, Wang S, Zhang R, Wei M, Liu W, Cao J, Zhang Y, Zhang Z, Xue T, Zheng Y, Song X
iScience 2023 Jan 20;26(1):105740

Heliangin acts as a covalent ligand of RPS2 that disrupts pre-rRNA metabolic processes in NPM1-mutated acute myeloid leukemia.

Feng Y, Han Y, Hu A, Qu Y, Hu Y, Wu H, Wang X, He L
Acta pharmaceutica Sinica. B 2023 Feb;13(2):598-617

Seven oxidative stress-related genes predict the prognosis of hepatocellular carcinoma.

Miao C, He X, Chen G, Kahlert UD, Yao C, Shi W, Su D, Hu L, Zhang Z
Aging 2023 Dec 14;15(24):15050-15063

GGC repeat expansion in NOTCH2NLC induces dysfunction in ribosome biogenesis and translation.

Fan Y, Li MJ, Yang J, Li SJ, Hao XY, Li JD, Wang YC, Tang MB, Zhang C, Shi JJ, Ma DR, Guo MN, Liu F, Shen S, Yao DB, Zuo CY, Mao CY, Hu ZW, Zhang S, Yang ZH, Guo GY, Yang JH, Xia ZP, Xu YM, Shi CH
Brain : a journal of neurology 2023 Aug 1;146(8):3373-3391

NOP53 undergoes liquid-liquid phase separation and promotes tumor radio-resistance.

Shi J, Chen SY, Shen XT, Yin XK, Zhao WW, Bai SM, Feng WX, Feng LL, Qin C, Zheng J, Wang YL, Fan XJ
Cell death discovery 2022 Oct 31;8(1):436

Downregulated RRS1 inhibits invasion and metastasis of BT549 through RPL11‑c‑Myc‑SNAIL axis.

Wang R, Peng C, Song J, Hua Y, Wu Q, Deng L, Cao Y, Zhang J, Zhang L, Wu L, Hou L
International journal of oncology 2022 Mar;60(3)

NPM1 promotes cell proliferation by targeting PRDX6 in colorectal cancer.

Wang D, Li Y, Liu Y, Cheng S, Liu F, Zuo R, Ding C, Shi S, Liu G
The international journal of biochemistry & cell biology 2022 Jun;147:106233

The binding between NPM and H2B proteins signals for the diabetes-associated centrosome amplification.

Xu SX, Han YW, Guo JL, Bian XK, Hu HM, Lee SC
Cell biochemistry and function 2022 Jul;40(5):516-525

Disruption of ER-mitochondria tethering and signalling in C9orf72-associated amyotrophic lateral sclerosis and frontotemporal dementia.

Gomez-Suaga P, Mórotz GM, Markovinovic A, Martín-Guerrero SM, Preza E, Arias N, Mayl K, Aabdien A, Gesheva V, Nishimura A, Annibali A, Lee Y, Mitchell JC, Wray S, Shaw C, Noble W, Miller CCJ
Aging cell 2022 Feb;21(2):e13549

Elevated FAM84B promotes cell proliferation via interacting with NPM1 in esophageal squamous cell carcinoma.

Wang F, Cheng C, Wang X, Chen F, Li H, Zhou Y, Wang Y, Hu X, Kong P, Zhang L, Cheng X, Cui Y
Cell death discovery 2022 Apr 8;8(1):182

Nuclear speckle specific hnRNP D-like prevents age- and AD-related cognitive decline by modulating RNA splicing.

Zhang Q, Zhang J, Ye J, Li X, Liu H, Ma X, Wang C, He K, Zhang W, Yuan J, Zhao Y, Xu H, Liu Q
Molecular neurodegeneration 2021 Sep 22;16(1):66

APOBEC1 complementation factor facilitates cell migration by promoting nucleus translocation of SMAD3 in renal cell carcinoma cells.

Xia H, Liu Y, Xu L, Mao H, Zhou Q, Xie Y
In vitro cellular & developmental biology. Animal 2021 May;57(5):501-509

HO-1 nuclear accumulation and interaction with NPM1 protect against stress-induced endothelial senescence independent of its enzymatic activity.

Luo W, Li J, Li Z, Lin T, Zhang L, Yang W, Mai Y, Liu R, Chen M, Dai C, Yang H, Lu J, Li H, Guan G, Huang M, Liu P, Li Z
Cell death & disease 2021 Jul 26;12(8):738

Genomic gain of RRS1 promotes hepatocellular carcinoma through reducing the RPL11-MDM2-p53 signaling.

Cao P, Yang A, Li P, Xia X, Han Y, Zhou G, Wang R, Yang F, Li Y, Zhang Y, Cui Y, Ji H, Lu L, He F, Zhou G
Science advances 2021 Aug;7(35)

Assessment of SENP3-interacting proteins in hepatocytes treated with diethylnitrosamine by BioID assay.

Chen F, Yan H, Guo C, Zhu H, Yi J, Sun X, Yang J
Acta biochimica et biophysica Sinica 2021 Aug 31;53(9):1237-1246

The protease activity of human ATG4B is regulated by reversible oxidative modification.

Zheng X, Yang Z, Gu Q, Xia F, Fu Y, Liu P, Yin XM, Li M
Autophagy 2020 Oct;16(10):1838-1850

Myc-mediated SDHA acetylation triggers epigenetic regulation of gene expression and tumorigenesis.

Li ST, Huang D, Shen S, Cai Y, Xing S, Wu G, Jiang Z, Hao Y, Yuan M, Wang N, Zhu L, Yan R, Yang D, Wang L, Liu Z, Hu X, Zhou R, Qu K, Li A, Duan X, Zhang H, Gao P
Nature metabolism 2020 Mar;2(3):256-269

Tight expression regulation of senataxin, linked to motor neuron disease and ataxia, is required to avert cell-cycle block and nucleolus disassembly.

Bennett CL, Sopher BL, La Spada AR
Heliyon 2020 Jun;6(6):e04165

MYCT1 represses apoptosis of laryngeal cancerous cells through the MAX/miR-181a/NPM1 pathway.

Wang HT, Tong X, Zhang ZX, Sun YY, Yan W, Xu ZM, Fu WN
The FEBS journal 2019 Oct;286(19):3892-3908

Identification of the key genes and pathways in prostate cancer.

Fan S, Liang Z, Gao Z, Pan Z, Han S, Liu X, Zhao C, Yang W, Pan Z, Feng W
Oncology letters 2018 Nov;16(5):6663-6669

Menin enhances c-Myc-mediated transcription to promote cancer progression.

Wu G, Yuan M, Shen S, Ma X, Fang J, Zhu L, Sun L, Liu Z, He X, Huang D, Li T, Li C, Wu J, Hu X, Li Z, Song L, Qu K, Zhang H, Gao P
Nature communications 2017 May 5;8:15278

Depletion of Dicer promotes epithelial ovarian cancer progression by elevating PDIA3 expression.

Zhu Y, Cai L, Guo J, Chen N, Yi X, Zhao Y, Cai J, Wang Z
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 Oct;37(10):14009-14023

A redox mechanism underlying nucleolar stress sensing by nucleophosmin.

Yang K, Wang M, Zhao Y, Sun X, Yang Y, Li X, Zhou A, Chu H, Zhou H, Xu J, Wu M, Yang J, Yi J
Nature communications 2016 Nov 25;7:13599

Nucleolar stress and impaired stress granule formation contribute to C9orf72 RAN translation-induced cytotoxicity.

Tao Z, Wang H, Xia Q, Li K, Li K, Jiang X, Xu G, Wang G, Ying Z
Human molecular genetics 2015 May 1;24(9):2426-41

Proteomic characterization of the nucleolar linker histone H1 interaction network.

Szerlong HJ, Herman JA, Krause CM, DeLuca JG, Skoultchi A, Winger QA, Prenni JE, Hansen JC
Journal of molecular biology 2015 Jun 5;427(11):2056-71

HP1-mediated formation of alternative lengthening of telomeres-associated PML bodies requires HIRA but not ASF1a.

Jiang WQ, Nguyen A, Cao Y, Chang AC, Reddel RR
PloS one 2011 Feb 15;6(2):e17036

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HepG2 cells were subjected to SDS PAGE followed by western blot with 60096-1-Ig(B23 antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of MCF-7 cells, using B23 antibody 60096-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human breast cancer using 60096-1-Ig(B23 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human breast cancer using 60096-1-Ig(B23 antibody) at dilution of 1:100 (under 40x lens)
Sample type: tissue