60096-1-Ig

antibody from Proteintech Group

Targeting: NPM1 B23, NPM

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

60096-1-Ig

Provider

Proteintech Group

Proper citation

Proteintech Cat#60096-1-Ig, RRID:AB_2155162

Product name

B23/NPM1 antibody

Antibody type

Monoclonal

Description

KD/KO validated B23/NPM1 antibody (Cat. #60096-1-Ig) is a mouse monoclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

4F12A3

Vial size

20ul, 150ul

References

Submitted references

Nuclear speckle specific hnRNP D-like prevents age- and AD-related cognitive decline by modulating RNA splicing.

Zhang Q, Zhang J, Ye J, Li X, Liu H, Ma X, Wang C, He K, Zhang W, Yuan J, Zhao Y, Xu H, Liu Q
Molecular neurodegeneration 2021 Sep 22;16(1):66

---

APOBEC1 complementation factor facilitates cell migration by promoting nucleus translocation of SMAD3 in renal cell carcinoma cells.

Xia H, Liu Y, Xu L, Mao H, Zhou Q, Xie Y
In vitro cellular & developmental biology. Animal 2021 May;57(5):501-509

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HO-1 nuclear accumulation and interaction with NPM1 protect against stress-induced endothelial senescence independent of its enzymatic activity.

Luo W, Li J, Li Z, Lin T, Zhang L, Yang W, Mai Y, Liu R, Chen M, Dai C, Yang H, Lu J, Li H, Guan G, Huang M, Liu P, Li Z
Cell death & disease 2021 Jul 26;12(8):738

---

Genomic gain of RRS1 promotes hepatocellular carcinoma through reducing the RPL11-MDM2-p53 signaling.

Cao P, Yang A, Li P, Xia X, Han Y, Zhou G, Wang R, Yang F, Li Y, Zhang Y, Cui Y, Ji H, Lu L, He F, Zhou G
Science advances 2021 Aug;7(35)

---

Assessment of SENP3-interacting proteins in hepatocytes treated with diethylnitrosamine by BioID assay.

Chen F, Yan H, Guo C, Zhu H, Yi J, Sun X, Yang J
Acta biochimica et biophysica Sinica 2021 Aug 31;53(9):1237-1246

---

The protease activity of human ATG4B is regulated by reversible oxidative modification.

Zheng X, Yang Z, Gu Q, Xia F, Fu Y, Liu P, Yin XM, Li M
Autophagy 2020 Oct;16(10):1838-1850

---

Myc-mediated SDHA acetylation triggers epigenetic regulation of gene expression and tumorigenesis.

Li ST, Huang, Shen S, Cai Y, Xing S, Wu G, Jiang Z, Hao Y, Yuan M, Wang N, Zhu L, Yan R, Yang D, Wang L, Liu Z, Hu X, Zhou R, Qu K, Li A, Duan X, Zhang H, Gao P
Nature metabolism 2020 Mar;2(3):256-269

---

Tight expression regulation of senataxin, linked to motor neuron disease and ataxia, is required to avert cell-cycle block and nucleolus disassembly.

Bennett CL, Sopher BL, La Spada AR
Heliyon 2020 Jun;6(6):e04165

---

MYCT1 represses apoptosis of laryngeal cancerous cells through the MAX/miR-181a/NPM1 pathway.

Wang HT, Tong X, Zhang ZX, Sun YY, Yan W, Xu ZM, Fu WN
The FEBS journal 2019 Oct;286(19):3892-3908

---

Identification of the key genes and pathways in prostate cancer.

Fan S, Liang Z, Gao Z, Pan Z, Han S, Liu X, Zhao C, Yang W, Pan Z, Feng W
Oncology letters 2018 Nov;16(5):6663-6669

---

Menin enhances c-Myc-mediated transcription to promote cancer progression.

Wu G, Yuan M, Shen S, Ma X, Fang J, Zhu L, Sun L, Liu Z, He X, Huang, Li T, Li C, Wu J, Hu X, Li Z, Song L, Qu K, Zhang H, Gao P
Nature communications 2017 May 5;8:15278

---

Depletion of Dicer promotes epithelial ovarian cancer progression by elevating PDIA3 expression.

Zhu Y, Cai L, Guo J, Chen N, Yi X, Zhao Y, Cai J, Wang Z
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 Oct;37(10):14009-14023

---

A redox mechanism underlying nucleolar stress sensing by nucleophosmin.

Yang K, Wang M, Zhao Y, Sun X, Yang Y, Li X, Zhou A, Chu H, Zhou H, Xu J, Wu M, Yang J, Yi J
Nature communications 2016 Nov 25;7:13599

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Nucleolar stress and impaired stress granule formation contribute to C9orf72 RAN translation-induced cytotoxicity.

Tao Z, Wang H, Xia Q, Li K, Li K, Jiang X, Xu G, Wang G, Ying Z
Human molecular genetics 2015 May 1;24(9):2426-41

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Proteomic characterization of the nucleolar linker histone H1 interaction network.

Szerlong HJ, Herman JA, Krause CM, DeLuca JG, Skoultchi A, Winger QA, Prenni JE, Hansen JC
Journal of molecular biology 2015 Jun 5;427(11):2056-71

---

HP1-mediated formation of alternative lengthening of telomeres-associated PML bodies requires HIRA but not ASF1a.

Jiang WQ, Nguyen A, Cao Y, Chang AC, Reddel RR
PloS one 2011 Feb 15;6(2):e17036

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 60096-1-Ig(B23 antibody) at dilution of 1:1000

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells, using B23 antibody 60096-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human breast cancer using 60096-1-Ig(B23 antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human breast cancer using 60096-1-Ig(B23 antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue