Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-36786 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-NPM1 (Thr234) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 38 kDa. Purity is >95% by SDS-PAGE.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-NPM1 (Thr234) in Lane 1: MCF-7 cell lysate treated with colchicine (0.2 µg/ML,24h), Lane 2: Raw264.7 cell treated with colchicine (0.2 µg/ML,24h), Lane 3: PC12 cell lysate treated with colchicine (0.2 µg/ML,24h). Samples were incubated with Phospho-NPM1 (Thr234) polyclonal antibody (Product # PA5-36786) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-NPM pThr234 using Phospho-NPM pThr234 polyclonal antibody (Product # PA5-36786) at a dilution of 1:500. Lane 1: MCF-7 cell lysate treated with colchicine (0.2 µg/mL, 24h), Lane 2: Raw264.7 cells treated with colchicine (0.2 µg/mL, 24h), Lane 3: PC12 cell lysate treated with colchicine (0.2 µg/mL, 24h).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Phospho-NPM1 (Thr234) in paraffin-embedded human kidney carcinoma tissue (nucleus staining) and negative control (right, with PBS only). Samples were incubated with Phospho-NPM1 (Thr234) polyclonal antibody (Product # PA5-36786) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Phospho-NPM1 (Thr234) in paraffin-embedded human breast carcinoma tissue (nucleus staining) and negative control (right, with PBS only). Samples were incubated with Phospho-NPM1 (Thr234) polyclonal antibody (Product # PA5-36786) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.