SM6037S

antibody from Acris Antibodies GmbH

Targeting: NPM1 B23, NPM

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

SM6037S

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#SM6037S, RRID:AB_1620381

Product name

anti Nucleophosmin

Antibody type

Monoclonal

Antigen

Recombinant human NPM (81-294 aa) purified from E. coli

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

5E3

Vial size

50 µl

Concentration

1.0 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysis: HeLa and HepG2 cell lysates (5 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SM6037 NPM antibody (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

The HeLa and HepG2 cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NPM (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry: SM6037 NPM antibody staining of Paraffin-Embedded Human Palatine Tonsil Tissue at 1/100 dilution.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry: SM6037 NPM antibody staining of Formalin-Fixed, Paraffin-Embedded Human Liver at 5 µg/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry: SM6037 NPM antibody staining of Formalin-Fixed, Paraffin-Embedded Human Skin at 5 µg/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

This image shows paraffin-embedded human palatine tonsil tissue sample stained with anti-NPM antibody (5E3) at 1:100 dilution.