Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- F48248 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- NPM1 Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 198-226 from the human protein was used as the immunogen for this NPM1 antibody.
- Description
- Purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the NPM1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- NPM1 antibody western blot analysis in 1) HeLa and 2) Jurkat lysate
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Fluorescent image of A549 cells stained with NPM1 antibody. Alexa Fluor 488 conjugated secondary was used (green). NPM1 immunoreactivity is localized to the nucleus and nucleolus significantly.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- NPM1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- NPM1 antibody flow cytometric analysis of HeLa cells (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.