Antibody data
- Antibody Data
- Antigen structure
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- Validations
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- Product number
- 686803 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#686803, RRID:AB_2617022
- Product name
- Alexa Fluor® 594 anti-Nucleophosmin
- Antibody type
- Monoclonal
- Antigen
- Purified recombinant human nucleophosmin (2-265 a.a.) expressed in E. coli.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Antibody clone number
- P87H10B9
- Vial size
- 25 µg
- Concentration
- 0.5 μ/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 5% FBS for 60 minutes. The cells were intracellularly stained with 1 µg/ml Alexa Fluor® 594 anti-Nucleophosmin antibody (clone P87H10B9) overnight at 4°C. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
- Conjugate
- Yellow dye