MA3-25200-A647

antibody from Invitrogen Antibodies

Targeting: NPM1 B23, NPM

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA3-25200-A647

Provider

Invitrogen Antibodies

Product name

NPM1 Monoclonal Antibody (FC-61991), Alexa Fluor™ 647

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Red dye

Isotype

IgG

Antibody clone number

FC-61991

Vial size

50 µL

Concentration

1 mg/mL

Storage

4° C, store in dark, DO NOT FREEZE!

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Nucleophosmin (red) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Nucleophosmin Monoclonal Antibody, AlexaFluor 647 conjugate (Product # MA3-25200-A647) at a dilution of 2.5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

Conjugate

Red dye