ABIN453229

antibody from antibodies-online

Targeting: NPM1 B23, NPM

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

ABIN453229

Provider

antibodies-online

Product name

anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (C-Term) antibody

Antibody type

Polyclonal

Antigen

KLH conjugated synthetic peptide selected from the C-terminal region of human NPM1

Reactivity

Human

Host

Rabbit

Vial size

0.1 mg

Concentration

0.25 mg/ml

Storage

Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

References

Submitted references

Detection of nucleophosmin gene mutations in plasma from patients with acute myeloid leukemia: clinical significance and implications.

Ma W, Kantarjian H, Zhang X, Jilani I, Sheikholeslami MR, Donahue AC, Ravandi F, Estey E, O'Brien S, Keating M, Giles FJ, Albitar M
Cancer biomarkers : section A of Disease markers 2009;5(1):51-8

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APE1/Ref-1 interacts with NPM1 within nucleoli and plays a role in the rRNA quality control process.

Vascotto C, Fantini D, Romanello M, Cesaratto L, Deganuto M, Leonardi A, Radicella JP, Kelley MR, D'Ambrosio C, Scaloni A, Quadrifoglio F, Tell G
Molecular and cellular biology 2009 Apr;29(7):1834-54

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B23/nucleophosmin serine 4 phosphorylation mediates mitotic functions of polo-like kinase 1.

Zhang H, Shi X, Paddon H, Hampong M, Dai W, Pelech S
The Journal of biological chemistry 2004 Aug 20;279(34):35726-34

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Western blot analysis of NPM1 (arrow) using rabbit polyclonal NPM1 Antibody (C-term) (AP17598PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NPM1 gene (Lane 2) (Origene Technologies).