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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- V7981 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Recombinant Nucleophosmin Antibody / NPM1
- Antibody type
- Monoclonal
- Description
- This highly specific Recombinant Nucleophosmin antibody is suitable for use in Immunohistochemistry/Western blot/Flow cytometry applications with human samples.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- rNPM1/1901
- Vial size
- 20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)
- Concentration
- 0.2 mg/ml, 1 mg/ml
- Storage
- Store the recombinant Nucleophosmin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human K562 cell lysate with recombinant Nucleophosmin antibody. Predicted molecular weight ~38 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human skin with recombinant Nucleophosmin antibody (clone rNPM1/1901). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human skin with recombinant Nucleophosmin antibody (clone rNPM1/1901). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of PFA-fixed human HeLa cells with recombinant Nucleophosmin antibody (clone rNPM1/1901); Red=isotype control, Blue= recombinant Nucleophosmin antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free recombinant Nucleophosmin antibody (clone rNPM1/1901) as confirmation of integrity and purity.
