AGR-026-25UL

antibody from Invitrogen Antibodies

Targeting: SCTR

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AGR-026-25UL

Provider

Invitrogen Antibodies

Product name

Secretin Receptor (extracellular) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

25 µL

Concentration

0.8 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of rat brain membranes (lanes 1 and 5), mouse brain membranes (lanes 2 and 6), rat pancreas lysate (lanes 3 and 7) and mouse kidney membranes (lanes 4 and 8): - 1-4. Anti-Secretin Receptor (extracellular) Antibody (#AGR-026), (1:200). 5-8. Anti-Secretin Receptor (extracellular) Antibody , preincubated with Secretin Receptor (extracellular) Blocking Peptide (#BLP-GR026).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human PANC-1 pancreatic carcinoma cell lysate: - 1. Anti-Secretin Receptor (extracellular) Antibody (#AGR-026), (1:200). 2. Anti-Secretin Receptor (extracellular) Antibody , preincubated with Secretin Receptor (extracellular) Blocking Peptide (#BLP-GR026).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of rat brain membranes (lanes 1 and 5), mouse brain membranes (lanes 2 and 6), rat pancreas lysate (lanes 3 and 7) and mouse kidney membranes (lanes 4 and 8): - 1-4. Anti-Secretin Receptor (extracellular) Antibody (#AGR-026), (1:200). 5-8. Anti-Secretin Receptor (extracellular) Antibody , preincubated with Secretin Receptor (extracellular) Blocking Peptide (#BLP-GR026).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of Secretin Receptor in mouse cerebellum - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Secretin Receptor (extracellular) Antibody (#AGR-026), (1:200), followed by goat Anti-rabbit-AlexaFluor-488. A. Secretin Receptor immunoreactivity (green) appears in Bergmann glia profiles (vertical arrows point at Bergmann cell bodies and horizontal arrows at Bergmann glia processes). B. Pre-incubation of the Antibody with Secretin Receptor (extracellular) Blocking Peptide (#BLP-GR026), suppresses staining. Cell nuclei are stained with DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of Secretin Receptor in rat hippocampus - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Secretin Receptor (extracellular) Antibody (#AGR-026), (1:200), followed by goat Anti-rabbit-AlexaFluor-488. A. Secretin Receptor immunoreactivity (green) appears in astrocyte profiles (arrows). G = granule layer. OML = outer molecular layer. B. Pre-incubation of the Antibody with Secretin Receptor (extracellular) Blocking Peptide (#BLP-GR026), suppresses staining. Cell nuclei are stained with DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of Secretin Receptor in mouse cerebellum - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Secretin Receptor (extracellular) Antibody (#AGR-026), (1:200), followed by goat Anti-rabbit-AlexaFluor-488. A. Secretin Receptor immunoreactivity (green) appears in Bergmann glia profiles (vertical arrows point at Bergmann cell bodies and horizontal arrows at Bergmann glia processes). B. Pre-incubation of the Antibody with Secretin Receptor (extracellular) Blocking Peptide (#BLP-GR026), suppresses staining. Cell nuclei are stained with DAPI (blue).