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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- ASR-090-50UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPR91 (SUCNR1) (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat kidney membrane (lanes 1 and 3), mouse kidney lysate (lanes 2 and 4), mouse TK-1 T-cell lymphoma (lanes 5 and 8), mouse J774 macrophage cell (lanes 6 and 9) and human Jurkat acute T cell leukemia (lanes 7 and 10) lysates: - 1,2,5,6,7. Anti-GPR91 (SUCNR1) (extracellular) Antibody (#ASR-090) (1:200).3,4,8,9,10. Anti-GPR91 (SUCNR1) (extracellular) Antibody , preincubated with GPR91/SUCNR1 (extracellular) Blocking Peptide (#BLP-SR090).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat kidney membrane (lanes 1 and 3), mouse kidney lysate (lanes 2 and 4), mouse TK-1 T-cell lymphoma (lanes 5 and 8), mouse J774 macrophage cell (lanes 6 and 9) and human Jurkat acute T cell leukemia (lanes 7 and 10) lysates: - 1,2,5,6,7. Anti-GPR91 (SUCNR1) (extracellular) Antibody (#ASR-090) (1:200).3,4,8,9,10. Anti-GPR91 (SUCNR1) (extracellular) Antibody , preincubated with GPR91/SUCNR1 (extracellular) Blocking Peptide (#BLP-SR090).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of GPR91 in mouse hippocampus. Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GPR91 (SUCNR1) (extracellular) Antibody (#ASR-090), (1:200), followed by goat Anti-rabbit-AlexaFluor-488. A. GPR91 immunoreactivity (green) appears in neuronal profiles in the pyramidal layer (P, vertical arrow) and the stratum oriens (SO, horizontal arrow). B. Pre-incubation of the Antibody with GPR91 (SUCNR1) (extracellular) Blocking Peptide (BLP-SR090), suppressed staining. Cell nuclei are stained with DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of GPR91 in mouse hippocampus. Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GPR91 (SUCNR1) (extracellular) Antibody (#ASR-090), (1:200), followed by goat Anti-rabbit-AlexaFluor-488. A. GPR91 immunoreactivity (green) appears in neuronal profiles in the pyramidal layer (P, vertical arrow) and the stratum oriens (SO, horizontal arrow). B. Pre-incubation of the Antibody with GPR91 (SUCNR1) (extracellular) Blocking Peptide (BLP-SR090), suppressed staining. Cell nuclei are stained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection ofGPR91 in live intact human THP-1 monocytic leukemia cells: - (black line) cells. (red) Cells + Goat Anti-rabbit-FITC. (green) Cells + Anti-GPR91 (SUCNR1) (extracellular) Antibody (#ASR-090), (2.5 μg/0.5x106 cells).
