A302-768A

antibody from Invitrogen Antibodies

Targeting: MRTFB FLJ31823, MKL2, MRTF-B

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A302-768A

Provider

Invitrogen Antibodies

Product name

MKL2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.20 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse MKL2 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 µg), and mouse NIH3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-MKL2 antibody A302-768A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. MKL2 was also immunoprecipitated by rabbit anti-MKL2 antibody A302-767A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human MKL2 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma and linitis plastica stomach cancer. Antibody: Affinity purified rabbit anti-MKL2 (Cat. No. A302-768A Lot1) used at a dilution of 1:200 (1µg/ml) and 1:1,000 (0.2µg/ml). Detection: DAB.