Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP1-46209 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-46209, RRID:AB_10009441
- Product name
- Rabbit Polyclonal MKL2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Phosphorylation of Npas4 by MAPK Regulates Reward-Related Gene Expression and Behaviors.
Tightly controlled MRTF-A activity regulates epithelial differentiation during formation of mammary acini.
Funahashi Y, Ariza A, Emi R, Xu Y, Shan W, Suzuki K, Kozawa S, Ahammad RU, Wu M, Takano T, Yura Y, Kuroda K, Nagai T, Amano M, Yamada K, Kaibuchi K
Cell reports 2019 Dec 3;29(10):3235-3252.e9
Cell reports 2019 Dec 3;29(10):3235-3252.e9
Tightly controlled MRTF-A activity regulates epithelial differentiation during formation of mammary acini.
Seifert A, Posern G
Breast cancer research : BCR 2017 Jun 7;19(1):68
Breast cancer research : BCR 2017 Jun 7;19(1):68
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MKL2 Antibody [NBP1-46209] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MKL2 antibody NBP1-46209 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. MKL2 was also immunoprecipitated by rabbit anti-MKL2 antibody NBP1-46208, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MKL2 Antibody [NBP1-46209] - Section of human prostate carcinoma and linitis plastica stomach cancer . Antibody: Affinity purified rabbit anti-MKL2 used at a dilution of 1:200 (1ug/ml) and 1:1,000 (0.2ug/ml). Detection: DAB