H00124540-M11

antibody from Abnova Corporation

Targeting: MSI2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00124540-M11

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00124540-M11, RRID:AB_581864

Product name

MSI2 monoclonal antibody (M11), clone 3B6

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant MSI2.

Antigen sequence

MEANGSQGTSGSANDSQHDPGKMFIGGLSWQTSPD
SLRDYFSKFGEIRECMVMRDPTTKRSRGFGFVTFA
DPASVDKVLGQPHHELDSKTIDPKVAFPRRAQPKM
VTRTKKIFVGGLSANTVVEDVKQYFEQFGKVEDAM
LMFDKTTNRHRGFGFVTFENEDVVEKVCEIHFHEI
NNKMVECKKAQPKEVMFPPGTRGRARGLPYTMDAF
MLGMGMLGYPNFVATYGRGYPGFAPSYGYQFPGFP
AAAYGPVAAAAVAAARGSGSNPARPGGFPGANSPG
PVADLYGPASQDSGVGNYISAASPQPGSGFGHGIA
GPLIATAFTNGYH


Isotype

IgG

Antibody clone number

3B6

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of MSI2 expression in transfected 293T cell line by MSI2 monoclonal antibody (M11), clone 3B6.Lane 1: MSI2 transfected lysate(35.2 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged MSI2 is 0.1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of MSI2 transfected lysate using anti-MSI2 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with MSI2 monoclonal antibody.

Validation comment

Immunoprecipitation

Protocol

Protocol