PA5-109206

antibody from Invitrogen Antibodies

Targeting: MSI2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-109206

Provider

Invitrogen Antibodies

Product name

MSI2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Control: SW480, K562, HepG2, PC-12, SH-SY5Y, rat epididymis tissue, human breast tissue, human placenta tissue, mouse brain tissue.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MSI2 in SW480 (1) and K562 (2) cell lysate. Samples were incubated with MSI2 polyclonal antibody (Product # PA5-109206), at a dilution of 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MSI2 in SH-SY5Y cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with MSI2 polyclonal antibody (Product # PA5-109206), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MSI2 in PC-12 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with MSI2 polyclonal antibody (Product # PA5-109206), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MSI2 in HepG2 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with MSI2 polyclonal antibody (Product # PA5-109206), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of MSI2 in paraffin-embedded rat epididymis tissue. Samples were incubated with MSI2 polyclonal antibody (Product # PA5-109206), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of MSI2 in paraffin-embedded mouse brain tissue. Samples were incubated with MSI2 polyclonal antibody (Product # PA5-109206), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of MSI2 in paraffin-embedded human placenta tissue. Samples were incubated with MSI2 polyclonal antibody (Product # PA5-109206), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of MSI2 in paraffin-embedded human breast tissue. Samples were incubated with MSI2 polyclonal antibody (Product # PA5-109206), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of MSI2 in SH-SY5Y cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with MSI2 polyclonal antibody (Product # PA5-109206) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti rabbit IgG.