PA5-44870

antibody from Invitrogen Antibodies

Targeting: DUSP5 HVH3

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-44870

Provider

Invitrogen Antibodies

Product name

DUSP5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA5-44870 detects DUSP5 in WB application.

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DUSP5 was performed by loading 35 µg of MiaPaCa-2 lysate in reducing sample buffer and Page Rµler Protein Ladder (Product # 26616) onto a 10% polyacrylamide gel. Proteins were transferred to PVDF membrane using the wet transfer method. Membrane was blocked in 5% milk. DUSP5 was detected at approximately 37 kDa using a DUSP5 polyclonal antibody (Product # PA5-44870) at a dilution of 1:500 in 5% milk, followed by a goat anti-rabbit IgG DyLight 800 conjugated secondary antibody (Product # SA5-35571) at a dilution of 1:6000. Data courtesy of Antibody Data Exchange Program.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human COLO205 cell lysate using an anti-DUSP5 polyclonal antibody (Product # PA5-44870).