LS-C287002

antibody from LSBio

Targeting: SMC3 BAM, bamacan, CSPG6, HCAP, SMC3L1

Western blot (Enhanced validation)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C287002

Provider

LSBio

Product name

SMC3 / HCAP Antibody (aa1175-C-term) LS-C287002

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Storage

Store at 2-8°C for up to one year.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of Human and Mouse SMC3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-SMC3 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of human and mouse SMC3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC3 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of Human SMC3 by Immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-SMC3 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of mouse SMC3 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-SMC3 used at 1:1,000 (1µg/ml). Detection: DAB

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of human SMC3 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-SMC3 used at 1:1,000 (1µg/ml). Detection: DAB