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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [2]
- Immunohistochemistry [1]
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- Product number
- GTX110402 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX110402, RRID:AB_1951949
- Product name
- SMC3 antibody [C2C3], C-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: PC-12 5% SDS PAGE GTX110402 diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: HepG2 (GTX27900) 5% SDS PAGE GTX110402 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 5% SDS PAGE GTX110402 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SMC3(GTX110402) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of SMC3 protein from HeLa whole cell extracts using 5 £gg of SMC3 antibody [C2C3], C-term (GTX110402).Western blot analysis was performed using SMC3 antibody [C2C3], C-term (GTX110402).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of SMC3 protein from HeLa whole cell extracts using 5 £gg of SMC3 antibody [C2C3], C-term (GTX110402) or SMC3 antibody [N1N2], N-term (GTX109827).Western blot analysis was performed using SMC3 antibody [C2C3], C-term (GTX110402).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using SMC3(GTX110402) antibody at 1:100 dilution.
