PA5-43068

antibody from Invitrogen Antibodies

Targeting: SMC3 BAM, bamacan, CSPG6, HCAP, SMC3L1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-43068

Provider

Invitrogen Antibodies

Product name

SMC3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide sequence: GKATLVMKKG DVEGSQSQDE GEGSGESERG SGSQSSVPSV DQFTGVGIRV

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human HepG2 cell lysate using an anti-SMC3 polyclonal antibody (Product # PA5-43068).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of human testis tissue using an anti-SMC3 polyclonal antibody (Product # PA5-43068).

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Chromatin Immunoprecipitation (ChIP) assay of endogenous SMC3 protein using Anti-SMC3 Antibody: ChIP was performed using Anti-SMC3 Rabbit Polyclonal Antibody (Product # PA5-43068, 5 µg) on sheared chromatin from HeLa cells using the MAGnify ChIP System kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR using primers binding to selected chromosomal positions: Chr 9 (11244 -11320), Chr 17 (7944729-794507) and SAT2 satellite repeats. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.