A300-060A

antibody from Invitrogen Antibodies

Targeting: SMC3 BAM, bamacan, CSPG6, HCAP, SMC3L1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A300-060A

Provider

Invitrogen Antibodies

Product name

SMC3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse SMC3 by western blot. Samples: Whole cell lysate (10 µg) from HEK293T, HeLa, Jurkat, LNCaP, U2OS, A-549, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC3 antibody (Product # A300-060A; lot A300-060A-10) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human SMC3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa, cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMC3 antibody A300-060A (lot A300-060A-9) used for IP at 3 µg per reaction. SMC3 was also immunoprecipitated by a previous lot of this antibody (lot A300-060A-8). For blotting immunoprecipitated SMC3, A300-060A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse SMC3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, Jurkat, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC3 antibody A300-060A (lot A300-060A-9) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse SMC3 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-SMC3 (Product # A300-060A; lot 10) used at 1:5,000 (0.2 µg/mL). Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human SMC3 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-SMC3 (Product # A300-060A lot 10) used at 1:5,000 (0.2 µg/mL). Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse SMC3 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-SMC3 Cat. No. A300-060A Lot9 used at 1:1,000 (1µg/ml). Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human SMC3 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-SMC3 Cat. No. A300-060A Lot9 used at 1:1,000 (1µg/ml). Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human SMC3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMC3 antibody (Product # A300-060A; lot A300-060A-10) used for IP at 6 µg per reaction. SMC3 was also immunoprecipitated by a previous lot of this antibody (lot A300-060A-9). For blotting immunoprecipitated SMC3 (Product # A300-060A) was used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.