Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP1-88715 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-88715, RRID:AB_11037640
- Product name
- Rabbit Polyclonal SMC3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human SMC3 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMC3 Antibody [NBP1-88715] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: U-251 MG
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: SMC3 Antibody [NBP1-88715] - Simple Western lane view shows a specific band for SMC3 in 0.2 mg/ml of U-251 MG lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation systems.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: SMC3 Antibody [NBP1-88715] - Electropherogram image of the corresponding Simple Western lane view. SMC3 antibody was used at 1:25 dilution on U-251 MG lysate(s) respectively.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: SMC3 Antibody [NBP1-88715] - Staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.