15676-1-AP

antibody from Proteintech Group

Targeting: HEXIM1 CLP-1, EDG1, HIS1, MAQ1

Provider product page for 15676-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [13]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunoprecipitation [1]
Immunohistochemistry [1]

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Product number: 15676-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#15676-1-AP, RRID:AB_2248363
Product name: HEXIM1 antibody
Antibody type: Polyclonal
Description: KD/KO validated HEXIM1 antibody (Cat. #15676-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

HEXIM1 protein structure - 15676-1-AP shown in red.

Submitted references Integrating cryo-OrbiSIMS with computational modelling and metadynamics simulations enhances RNA structure prediction at atomic resolution.

Ward S, Childs A, Staley C, Waugh C, Watts JA, Kotowska AM, Bhosale R, Borkar AN
Nature communications 2024 May 22;15(1):4367

SHIFTR enables the unbiased identification of proteins bound to specific RNA regions in live cells.

Aydin J, Gabel A, Zielinski S, Ganskih S, Schmidt N, Hartigan CR, Schenone M, Carr SA, Munschauer M
Nucleic acids research 2024 Mar 21;52(5):e26

N(6)-methyladenosine in 7SK small nuclear RNA underlies RNA polymerase II transcription regulation.

Wang Y, Traugot CM, Bubenik JL, Li T, Sheng P, Hiers NM, Fernandez P, Li L, Bian J, Swanson MS, Xie M
Molecular cell 2023 Nov 2;83(21):3818-3834.e7

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Burda JE, O'Shea TM, Ao Y, Suresh KB, Wang S, Bernstein AM, Chandra A, Deverasetty S, Kawaguchi R, Kim JH, McCallum S, Rogers A, Wahane S, Sofroniew MV
Nature 2022 Jun;606(7914):557-564

cAMP-Induced Nuclear Condensation of CRTC2 Promotes Transcription Elongation and Cystogenesis in Autosomal Dominant Polycystic Kidney Disease.

Mi Z, Song Y, Wang J, Liu Z, Cao X, Dang L, Lu Y, Sun Y, Xiong H, Zhang L, Chen Y
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2022 Apr;9(10):e2104578

Interaction of the Ankyrin H Core Effector of Legionella with the Host LARP7 Component of the 7SK snRNP Complex.

Von Dwingelo J, Chung IYW, Price CT, Li L, Jones S, Cygler M, Abu Kwaik Y
mBio 2019 Aug 27;10(4)

HMGA1 recruits CTIP2-repressed P-TEFb to the HIV-1 and cellular target promoters.

Eilebrecht S, Le Douce V, Riclet R, Targat B, Hallay H, Van Driessche B, Schwartz C, Robette G, Van Lint C, Rohr O, Benecke AG
Nucleic acids research 2014 Apr;42(8):4962-71

Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations.

Neumann M, Valori CF, Ansorge O, Kretzschmar HA, Munoz DG, Kusaka H, Yokota O, Ishihara K, Ang LC, Bilbao JM, Mackenzie IR
Acta neuropathologica 2012 Nov;124(5):705-16

Cardiac lineage protein-1 (CLP-1) regulates cardiac remodeling via transcriptional modulation of diverse hypertrophic and fibrotic responses and angiotensin II-transforming growth factor β (TGF-β1) signaling axis.

Mascareno E, Galatioto J, Rozenberg I, Salciccioli L, Kamran H, Lazar JM, Liu F, Pedrazzini T, Siddiqui MA
The Journal of biological chemistry 2012 Apr 13;287(16):13084-93

7SK snRNA-mediated, gene-specific cooperativity of HMGA1 and P-TEFb.

Eilebrecht S, Benecke BJ, Benecke A
RNA biology 2011 Nov-Dec;8(6):1084-93

CLP-1 associates with MyoD and HDAC to restore skeletal muscle cell regeneration.

Galatioto J, Mascareno E, Siddiqui MA
Journal of cell science 2010 Nov 1;123(Pt 21):3789-95

Positive transcription elongation factor b activity in compensatory myocardial hypertrophy is regulated by cardiac lineage protein-1.

Espinoza-Derout J, Wagner M, Salciccioli L, Lazar JM, Bhaduri S, Mascareno E, Chaqour B, Siddiqui MA
Circulation research 2009 Jun 19;104(12):1347-54

Pivotal role of cardiac lineage protein-1 (CLP-1) in transcriptional elongation factor P-TEFb complex formation in cardiac hypertrophy.

Espinoza-Derout J, Wagner M, Shahmiri K, Mascareno E, Chaqour B, Siddiqui MA
Cardiovascular research 2007 Jul 1;75(1):129-38

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 15676-1-AP(HEXIM1 antibody) at dilution of 1:1500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of MCF-7 cells, using HEXIM1 antibody 15676-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: IP Result of anti-HEXIM1 (IP:15676-1-AP, 4ug; Detection:15676-1-AP 1:500) with MCF-7 cells lysate 2000ug.
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human breast cancer using 15676-1-AP(HEXIM1 antibody) at dilution of 1:50 (under 40x lens)
Sample type: tissue